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Titolo:
THE CYSTEINE RESIDUES OF THE M2 PROTEIN ARE NOT REQUIRED FOR INFLUENZA-A VIRUS-REPLICATION
Autore:
CASTRUCCI MR; HUGHES M; CALZOLETTI L; DONATELLI I; WELLS K; TAKADA A; KAWAOKA Y;
Indirizzi:
UNIV WISCONSIN,SCH VET MED,DEPT PATHOBIOL SCI,2015 LINDEN DR W MADISON WI 53706 IST SUPER SANITA,DEPT VIROL I-00161 ROME ITALY ST JUDE CHILDRENS HOSP,DEPT VIROL & MOL BIOL MEMPHIS TN 38101 UNIV TENNESSEE,DEPT PATHOL MEMPHIS TN 38163 HOKKAIDO UNIV,GRAD SCH VET MED,DEPT DIS CONTROL,MICROBIOL LAB SAPPOROHOKKAIDO 060 JAPAN
Titolo Testata:
Virology
fascicolo: 1, volume: 238, anno: 1997,
pagine: 128 - 134
SICI:
0042-6822(1997)238:1<128:TCROTM>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC MUTAGENESIS; ION-CHANNEL ACTIVITY; A VIRUS; ENVELOPE GLYCOPROTEIN; MEMBRANE-FUSION; M(2) PROTEIN; SUBUNIT INTERACTIONS; CYTOPLASMIC TAIL; FATTY-ACIDS; HEMAGGLUTININ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
M.R. Castrucci et al., "THE CYSTEINE RESIDUES OF THE M2 PROTEIN ARE NOT REQUIRED FOR INFLUENZA-A VIRUS-REPLICATION", Virology, 238(1), 1997, pp. 128-134

Abstract

The M2 protein of influenza A virus functions as an ion channel. It contains three cysteine residues: cysteines 17 and 19, which form disulfide bonds in the ectodomain, and cysteine 50, which is acylated. To understand the role of these cysteine residues in virus replication, weused reverse genetics to create influenza viruses in which the individual cysteines were mutated and a virus in which all three cysteines were changed to serine. The M2 cysteine mutants that lacked either of the cysteine residues in the ectodomain and the mutant that lacked all three residues had appreciably lower amounts of M2 oligomers than did the wild-type virus when examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. None of the mutants, however, were defectivein replication, either in vitro or in ferrets and mice; These findings demonstrate that noncovalent interactions are sufficient for the M2 protein to form functional oligomers for virus replication and that its cysteine residues are dispensable for influenza virus replication invitro and in vivo. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:29:42