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Titolo:
HIPPOCAMPAL INTERNEURONS ARE EXCITED VIA SEROTONIN-GATED ION CHANNELS
Autore:
MCMAHON LL; KAUER JA;
Indirizzi:
DUKE UNIV,MED CTR,DEPT NEUROBIOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT NEUROBIOL DURHAM NC 27710
Titolo Testata:
Journal of neurophysiology
fascicolo: 5, volume: 78, anno: 1997,
pagine: 2493 - 2502
SICI:
0022-3077(1997)78:5<2493:HIAEVS>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACUNOSUM-MOLECULARE INTERNEURONS; MOUSE CENTRAL NEURONS; 5-HT3 RECEPTORS; RAT HIPPOCAMPUS; PATCH-CLAMP; INHIBITORY INTERNEURONS; DIVALENT-CATIONS; XENOPUS-OOCYTES; BLASTOMA CELLS; CA1 REGION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
L.L. Mcmahon e J.A. Kauer, "HIPPOCAMPAL INTERNEURONS ARE EXCITED VIA SEROTONIN-GATED ION CHANNELS", Journal of neurophysiology, 78(5), 1997, pp. 2493-2502

Abstract

Serotonergic neurons of the median raphe nucleus heavily innervate hippocampal GABAergic interneurons located in stratum radiatum of area CA1, suggesting that this strong subcortical projection may modulate interneuron excitability. Using whole cell patch-clamp recording from interneurons in brain slices, we tested the effects of serotonin (5-HT) on the physiological properties of these interneurons. Serotonin produces a rapid inward current that persists when synaptic transmission isblocked by tetrodotoxin and cobalt, and is unaffected by ionotropic glutamate and gamma-aminobutyric acid (GABA) receptor antagonists. The 5-HT-induced current was independent of G-protein activation. Pharmacological evidence indicates that 5-HT directly excites these interneurons through activation of 5-HT3 receptors. At membrane potentials negative to -55 mV, the current-voltage (I-V) relationship of the 5-HT current displays a region of negative slope conductance. Therefore the response of interneurons to 5-HT strongly depends on membrane potential and increases greatly as cells are depolarized. Removal of extracellular calcium, but not magnesium, increases the amplitude of 5-HT-induced currents and removes the region of negative slope conductance, therebylinearizing the I-V relationship. The axons of 5-HT-responsive interneurons ramify widely within CA1; some of these interneurons also project to and arborize extensively in the dentate gyrus. The organization of these inhibitory connections strongly suggests that these cells regulate excitability of both CA1 pyramidal cells and dentate granule cells. As our results indicate that 5-HT may mediate fast excitatory synaptic transmission onto these interneurons, serotonergic inputs can simultaneously modulate the output of both hippocampus and dentate gyrus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 17:31:00