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Titolo:
CONSTRUCTION AND ENHANCED CYTOTOXICITY OF A [CYANOVIRIN-N]-[PSEUDOMONAS EXOTOXIN] CONJUGATE AGAINST HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CELLS
Autore:
MORI T; SHOEMAKER RH; MCMAHON JB; GULAKOWSKI RJ; GUSTAFSON KR; BOYD MR;
Indirizzi:
NCI,LAB DRUG DISCOVERY RES & DEV,DEV THERAPEUT PROGRAM,DIV CANC TREATMENT DIAG & CTR,BLDG 1052 FREDERICK MD 21702 NCI,LAB DRUG DISCOVERY RES & DEV,DEV THERAPEUT PROGRAM,DIV CANC TREATMENT DIAG & CTR FREDERICK MD 21702
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 239, anno: 1997,
pagine: 884 - 888
SICI:
0006-291X(1997)239:3<884:CAECOA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT SOLUBLE CD4; HTLV-III/LAV ENVELOPE; PSEUDOMONAS EXOTOXIN; PHASE-I; HIV; TYPE-1; AIDS; NEUTRALIZATION; GLYCOPROTEIN; RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
T. Mori et al., "CONSTRUCTION AND ENHANCED CYTOTOXICITY OF A [CYANOVIRIN-N]-[PSEUDOMONAS EXOTOXIN] CONJUGATE AGAINST HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CELLS", Biochemical and biophysical research communications, 239(3), 1997, pp. 884-888

Abstract

Cyanovirin-N (CV-N) is a novel 11-kDa anti-HIV(human immunodeficiencyvirus) protein that binds with high affinity to the viral envelope glycoprotein gp120, in contrast to soluble CD4 and most known neutralizing antibodies that bind gp120, CV-N exerts potent anti-viral activity against primary clinical HN isolates as well as laboratory-adapted strains of HIV. Here we describe the recombinant production, purification, and characterization of a chimeric toxin molecule, FLAG-CV-N-PE38, that contains CV-N as a gp120-targeting moiety linked to the translocation and cytotoxic domains of Pseudomonas exotoxin A. FLAG-CV-N-PE38 showed enhanced cytotoxicity to HIV-infected, gp120-expressing H9 cells compared to uninfected H9 cells. Competition experiments with free CV-N provided further support that the enhanced FLAG-CV-N-PE38-induced cytotoxicity was due to interactions of the CV-N moiety with cell surface gp120. This study establishes the feasibility of use of CV-N as a gp120-targeting sequence for construction and experimental therapeutic investigations of unique new chimeric toxins designed to selectively destroy HIV-infected host cells. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:28:34