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Titolo:
THE MUTAGENIC RESPONSE AT THE OUABAIN RESISTANCE LOCUS IN T-CELLS OF MICE EXPOSED TO N-ETHYL-N-NITROSOUREA PARALLELS THE RESPONSE AT THE HPRT LOCUS AND CORRELATES WITH MUTATION TARGET SIZE
Autore:
DASS SB; HEFLICH RH; CASCIANO DA;
Indirizzi:
NATL CTR TOXICOL RES,DIV GENET & REPROD TOXICOL JEFFERSON AR 72079
Titolo Testata:
Carcinogenesis
fascicolo: 11, volume: 18, anno: 1997,
pagine: 2233 - 2237
SICI:
0143-3334(1997)18:11<2233:TMRATO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-SEQUENCE ANALYSIS; HAMSTER OVARY CELLS; CHINESE-HAMSTER; IN-VIVO; THIOGUANINE-RESISTANT; RELATIVE SENSITIVITY; CHO CELLS; LYMPHOCYTES; MOUSE; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
S.B. Dass et al., "THE MUTAGENIC RESPONSE AT THE OUABAIN RESISTANCE LOCUS IN T-CELLS OF MICE EXPOSED TO N-ETHYL-N-NITROSOUREA PARALLELS THE RESPONSE AT THE HPRT LOCUS AND CORRELATES WITH MUTATION TARGET SIZE", Carcinogenesis, 18(11), 1997, pp. 2233-2237

Abstract

The lymphocyte Hprt gene has been used extensively as a reporter locus to monitor the mutational effects of the exposure of animals to genotoxicants, Implicit in this view of the function of a reporter gene isthe assumption that its mutagenic response is representative of that of other genes in the organism, As a test of this hypothesis we compared the frequency of 6-thioguanine-resistant (TG(r)) mutants at the Hprt locus with the mutant frequency (MF) induced at another locus, the ouabain resistance (Oua) locus, The frequency of spontaneous OUA(R) mutants was estimated to be 1.1x10(-7) (MF between <0.3 and 1.1x10(-7)), which was similar to 30-fold less than the spontaneous TG(r) MF. Following treatment with N-ethyl-N-nitrosourea (ENU), the induced OUA(R) MFat each of two dose levels (50 and 150 mg/kg ENU) and two time points(3 and 6 weeks postexposure) was consistently 8- to 9-fold lower thanthe corresponding TG(r) MF. Thus the mutagenic response of the Oua locus closely paralleled that of the Hprt locus, indicating a similarityin their response to ENU, In addition, the Oua locus was 3-4 times more sensitive than the Hprt locus to the mutagenic effect of ENU, as measured by the fold increase in MF over the background level. The number of ENU-mutable sites capable of resulting in a TG(r) or OUA(R) phenotype, otherwise known as the mutation target size, was estimated to differ by an order of magnitude between the two loci, This difference intarget size correlates with, and therefore may largely account for, the difference in induced MF between both loci.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:26:22