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Titolo:
CASPASE-MEDIATED APOPTOSIS IN AK-5 TUMOR-CELLS - A CELL-FREE STUDY USING PEPTIDE INHIBITORS AND ANTISENSE STRATEGY
Autore:
ANJUM R; KHAR A;
Indirizzi:
CTR CELLULAR & MOL BIOL HYDERABAD 500007 ANDHRA PRADESH INDIA CTR CELLULAR & MOL BIOL HYDERABAD 500007 ANDHRA PRADESH INDIA
Titolo Testata:
Experimental cell research
fascicolo: 2, volume: 236, anno: 1997,
pagine: 371 - 377
SICI:
0014-4827(1997)236:2<371:CAIAT->2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEATH GENE CED-3; POLY(ADP-RIBOSE) POLYMERASE; PROTEIN-KINASE; IL-1-BETA-CONVERTING ENZYME; MAMMALIAN HOMOLOG; DNA FRAGMENTATION; RAT HISTIOCYTOMA; BCL-2; INDUCTION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
R. Anjum e A. Khar, "CASPASE-MEDIATED APOPTOSIS IN AK-5 TUMOR-CELLS - A CELL-FREE STUDY USING PEPTIDE INHIBITORS AND ANTISENSE STRATEGY", Experimental cell research, 236(2), 1997, pp. 371-377

Abstract

An in vitro system has been employed to study the apoptotic mechanisms in the AK-5 tumor which is a spontaneously regressing rat histiocytoma. Cytosolic extracts of tumor cells primed for apoptosis using dexamethasone and immune serum from tumor-regressing animals were able to induce apoptosis in intact nuclei and reproduce the classical morphological and biochemical features typical of apoptotic cells. The cleavageof lamin A and PARP to signature fragments by these extracts and the inhibition of the same using peptide inhibitors signify the pivotal role of ICE and ICE-related proteases in apoptosis. Lamin A cleavage wasinsensitive to YVAD but PARP cleavage was blocked by both YVAD and DEVD. Cell extracts derived from cells overexpressing the Bcl-2 gene andNedd-2 antisense gene, respectively, failed to induce apoptosis in exogenously added nuclei, suggesting that Bcl-2 gene product is downregulating a key event in apoptotic cascade. The study also demonstrates the coherent action of different ICE-related proteases in apoptosis andtheir functional redundancy. This system may prove useful for analyzing complex molecular mechanisms underlying apoptosis in tumor cells. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:55:29