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Titolo:
THE INFLUENCE OF HEMIN LEVELS ON GROWTH AND ENZYME-PRODUCTION BY PORPHYROMONAS-GINGIVALIS IN CONTINUOUS-CULTURE
Autore:
MINHAS T; GREENMAN J; SCHAFFER AG;
Indirizzi:
UNIV W ENGLAND,DEPT BIOL SCI,COLDHARBOUR LANE BRISTOL BS16 1QY ENGLAND UNIV W ENGLAND,DEPT BIOL SCI,COLDHARBOUR LANE BRISTOL BS16 1QY ENGLAND
Titolo Testata:
Microbios
fascicolo: 307, volume: 76, anno: 1993,
pagine: 105 - 114
SICI:
0026-2633(1993)76:307<105:TIOHLO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRYPSIN-LIKE PROTEASE; BACTEROIDES-GINGIVALIS; VIRULENCE FACTORS; W50; HEMIN; MELANINOGENICUS; PROTEINS; VESICLES;
Keywords:
PORPHYROMONAS-GINGIVALIS; HEMIN; HYDROLYTIC ENZYME PRODUCTION; CONTINUOUS CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
T. Minhas et al., "THE INFLUENCE OF HEMIN LEVELS ON GROWTH AND ENZYME-PRODUCTION BY PORPHYROMONAS-GINGIVALIS IN CONTINUOUS-CULTURE", Microbios, 76(307), 1993, pp. 105-114

Abstract

Porphyromonas gingivalis was grown in continuous culture in brain heart infusion medium with haemin (5 mug l-1 to 10 mg l-1). Growth was haemin-limited up to 400 mug l-1, and steady-state growth could not be obtained in the absence of haemin. It was confirmed that protoporphyrinIX could replace the haemin requirement. The results indicate that P.gingivalis has more than one uptake system for haemin, one of which is high affinity. The effect of haemin on hydrolytic enzyme production revealed a complex picture. Trypsin-like protease activity peaked at 200 mug l-1 with a substantial and highly significant reduction at high(2 and 10 mg l-1) concentrations of haemin. Alkaline phosphatase alsopeaked around 100 to 400 mug l-1 while N-acetyl-beta-glucosaminidase was not greatly affected except that a greater proportion of enzyme was secreted into the medium at the lowest haemin concentration. In general, haemin-limitation enhanced export of the hydrolytic enzymes to the extracellular vesicle fraction. Protoporphyrin IX at 2 mg l-1 causeda highly significant reduction in the production of all three enzymes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:52:13