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Titolo:
STAPHYLOCOCCAL SERINE PROTEINASE INCREASES INTRACELLULAR FREE CALCIUM-CONCENTRATION IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES
Autore:
NOWAK D; KROL M; MIEDZOBRODZKI J; BIALASIEWICZ P;
Indirizzi:
MED UNIV LODZ,DEPT PNEUMOL & ALLERGOL,KOPCINSKIEGO ST 22 PL-90153 LODZ POLAND JAGIELLONIAN UNIV,INST MOL BIOL,DEPT MICROBIOL & IMMUNOL PL-31120 KRAKOW POLAND
Titolo Testata:
Antonie van Leeuwenhoek
fascicolo: 3, volume: 72, anno: 1997,
pagine: 245 - 249
SICI:
0003-6072(1997)72:3<245:SSPIIF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA ALPHA-1-PROTEINASE INHIBITOR; METHIONYL-LEUCYL-PHENYLALANINE; CHEMOATTRACTANT ACTIVITY; AUREUS; INACTIVATION; NEUTROPHILS; INVITRO; CELLS;
Keywords:
STAPHYLOCOCCAL SERINE PROTEINASE; POLYMORPHONUCLEAR LEUKOCYTES; INTRACELLULAR CALCIUM; INTRACELLULAR CALCIUM STORES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
D. Nowak et al., "STAPHYLOCOCCAL SERINE PROTEINASE INCREASES INTRACELLULAR FREE CALCIUM-CONCENTRATION IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES", Antonie van Leeuwenhoek, 72(3), 1997, pp. 245-249

Abstract

Staphylococcal serine proteinase (SSP) can influence various functions of human polymorphonuclear leukocytes (PMNL) including chemotaxis and phagocytosis. Since the rise in intracellular free calcium concentration is an important step in signal transduction leading to phagocyte activation, we tested the ability of SSP to increase the intracellularfree calcium concentration in human PMNL using the fluorescent calcium indicator Fura-2AM. PMNL isolated from healthy donors responded to SSP in the concentration range of 10 to 100 mu g/ml. The highest Ca2+ rise (104 +/- 47 nM) was observed for 10 mu g/ml SSP. It was mainly dependent (81 +/- 11%) on extracellular calcium influx, however, SSP mobilized 68 +/- 7% of Ca2+ from intracellular calcium stores. Boiling of SSP or preincubation with phenylmethylsulphonylfluoride (an serine proteinase inhibitor) did not change its ability to increase intracellular free calcium concentration in PMNL. It suggests that active center of SSP is not responsible for Ca2+ mobilization. Finally, PMNL responded to each of three consecutive stimulations with SSP independently of the presence of high or low extracellular Ca2+ concentration. This maybe an additional mechanism responsible for activation of human PMNL and degradation of alveolar walls during the staphylococcal infection in the lower airways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 07:59:31