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Titolo:
BOTH CYCLING AND NONCYCLING PRIMITIVE PROGENITORS CONTINUE TO BE MOBILIZED INTO THE CIRCULATION DURING THE LEUKAPHERESIS OF PATIENTS PRETREATED WITH CHEMOTHERAPY AND G-CSF
Autore:
PONCHIO L; EAVES C; HOGGE D; PEROTTI C; TORRETTA L; CERANI P; SALVANESCHI L; ASCARI E; CAZZOLA M;
Indirizzi:
UNIV PAVIA,POLICLIN SAN MATTEO,IRCCS I-27100 PAVIA ITALY UNIV PAVIA,POLICLIN SAN MATTEO,IRCCS I-27100 PAVIA ITALY UNIV PAVIA,DEPT INTERNAL MED & MED THERAPY,SECT INTERNAL MED & MED ONCOL,SCH MED I-27100 PAVIA ITALY UNIV PAVIA,DEPT IMMUNOHAEMATOL & BLOOD TRANSFUS,SCH MED I-27100 PAVIAITALY BRITISH COLUMBIA CANC AGCY,TERRY FOX LAB VANCOUVER BC V5Z 1L3 CANADA
Titolo Testata:
British Journal of Haematology
fascicolo: 2, volume: 99, anno: 1997,
pagine: 394 - 402
SICI:
0007-1048(1997)99:2<394:BCANPP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM-CULTURE; COLONY-STIMULATING FACTOR; STEM-CELL TRANSPLANTATION; HUMAN HEMATOPOIETIC-CELLS; HUMAN PERIPHERAL-BLOOD; CHRONIC MYELOID-LEUKEMIA; INITIATING CELLS; FUNCTIONAL-CHARACTERIZATION; MARROW TRANSPLANTATION; CD34(+) CELLS;
Keywords:
HEMATOPOIESIS; CHEMOTHERAPY; GROWTH FACTORS; LEUKAPHERESIS; STEM CELL TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
L. Ponchio et al., "BOTH CYCLING AND NONCYCLING PRIMITIVE PROGENITORS CONTINUE TO BE MOBILIZED INTO THE CIRCULATION DURING THE LEUKAPHERESIS OF PATIENTS PRETREATED WITH CHEMOTHERAPY AND G-CSF", British Journal of Haematology, 99(2), 1997, pp. 394-402

Abstract

Colony-forming cells (CFC) and long-term culture-initiating cells (LTC-IC) include a spectrum of progenitor types whose potential contributions to the haemopoietic recovery seen in patients transplanted with mobilized peripheral blood progenitor cells (PBPC) remains unclear. We evaluated both the number and cycling status of the circulating LTC-ICand CFC harvested from 12 patients treated with chemotherapy and G-CSF using a modified 6-week LTC-IC assay, The frequency of the LTC-IC and CFC in the mobilized PB samples were increased 45- and 750-fold, respectively. Interestingly, comparison of these values for PB samples, taken just prior to the start of the leukapheresis, with the progenitorcontent of the 3 h harvest, showed that, on average, the leukapheresis product contained 19 times more LTC-IC (P<0.01) than had been detectable in the entire blood volume of the patients at the start of the collection, whereas the number of CFC collected was approximately the same as the number in the initial circulating pool of PBPC. Cycling studies showed many of the LTC-IC in the apheresis collections to be proliferating although not more so than in the steady-state marrow LTC-IC compartment (i.e. per cent kill of mobilized LTC-IC after 16 h in H-3-Tdr = 70 +/- 8%, n = 9), On the other hand, the majority of the CFC in the apheresis collections were initially quiescent (per cent kill after 16 h in H-3-Tdr = 37 +/- 6%, n = 12). These findings demonstrate therapidity with which a primitive subset of LTS-IC may enter the circulation during the early phase of rebound haemopoiesis induced by chemotherapy plus G-CSF and provide evidence of differences in the mechanisms regulating LTC-IC and CFC mobilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:22:50