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Titolo:
EVIDENCE AGAINST AN ACUTE INHIBITORY ROLE OF NSEC-1 (MUNC-18) IN LATESTEPS OF REGULATED EXOCYTOSIS IN CHROMAFFIN AND PC12 CELLS
Autore:
GRAHAM ME; SUDLOW AW; BURGOYNE RD;
Indirizzi:
UNIV LIVERPOOL,PHYSIOL LAB,CROWN ST LIVERPOOL L69 3BX MERSEYSIDE ENGLAND UNIV LIVERPOOL,PHYSIOL LAB LIVERPOOL L69 3BX MERSEYSIDE ENGLAND
Titolo Testata:
Journal of neurochemistry
fascicolo: 6, volume: 69, anno: 1997,
pagine: 2369 - 2377
SICI:
0022-3042(1997)69:6<2369:EAAAIR>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-DEPENDENT EXOCYTOSIS; INTEGRAL MEMBRANE-PROTEIN; SYNTAXIN-BINDING PROTEIN; SYNAPTIC VESICLE DOCKING; ALPHA-SNAP; NEUROTRANSMITTER RELEASE; CAENORHABDITIS-ELEGANS; VESICULAR TRANSPORT; FUSION PROTEIN; YEAST SEC1;
Keywords:
NSEC-1 (MUNC-18) PROTEIN; EXOCYTOSIS; CHROMAFFIN CELLS; PC12 CELLS; ALPHA-SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN ATTACHMENTPROTEIN; GROWTH HORMONE SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
M.E. Graham et al., "EVIDENCE AGAINST AN ACUTE INHIBITORY ROLE OF NSEC-1 (MUNC-18) IN LATESTEPS OF REGULATED EXOCYTOSIS IN CHROMAFFIN AND PC12 CELLS", Journal of neurochemistry, 69(6), 1997, pp. 2369-2377

Abstract

nSec-1 (munc-18) is a mammalian homologue of proteins implicated in constitutive exocytosis in yeast and neurotransmission in Caenorhabditis elegans and Drosophila, Mutant phenotypes in these species suggest that nSec-1 is likely to be required for neurotransmission. Various other data have been interpreted as suggesting that nSec-1 could also be a negative regulator of Ca2+-dependent exocytosis. We have tested thispossibility by introducing exogenous nSec-1 into permeabilised chromaffin or PC12 cells and examining its effects on Ca2+-induced and alpha-soluble N-ethylmaleimide-sensitive fusion protein attachment protein-stimulated exocytosis. No effects of exogenous nSec-1 were observed inthese assays. in addition, the effect of nSec-1 overexpression in transiently transfected PC12 cells on reporter growth hormone (GH) secretion was examined, Overexpression of nSec-1 resulted in a marked increase in GH production, reflected in an increase in both cell-associated and medium GH levels. The relative amounts retained in the cells were unaffected by nSec-1 overexpression, indicating that GH storage was unaffected and that the major effect was on its synthesis. In contrast, nSec-1 overexpression did not affect the proportion of GH that was released following stimulation in intact or permeabilised cells. These results suggest either that nSec-1 is already expressed at sufficient levels and remains so following permeabilisation or that nSec-1 may not be an acute inhibitory regulator of Ca2+-dependent exocytosis in chromaffin or PC12 cells.

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Documento generato il 07/07/20 alle ore 05:47:02