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Titolo:
CENTROSOME AND MICROTUBULE DYNAMICS IN APICAL CELLS OF SPHACELARIA-RIGIDULA (PHAEOPHYCEAE) TREATED WITH NOCODAZOLE
Autore:
KARYOPHYLLIS D; GALATIS B; KATSAROS C;
Indirizzi:
UNIV ATHENS,DIV BOT,DEPT BIOL GR-15784 ATHENS GREECE UNIV ATHENS,DIV BOT,DEPT BIOL GR-15784 ATHENS GREECE
Titolo Testata:
Protoplasma
fascicolo: 3-4, volume: 199, anno: 1997,
pagine: 161 - 172
SICI:
0033-183X(1997)199:3-4<161:CAMDIA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMOBASIDIOMYCETE SCHIZOPHYLLUM-COMMUNE; SPINDLE ASSEMBLY CHECKPOINT; HAMSTER OVARY CELLS; IMMUNOFLUORESCENCE MICROSCOPY; PHYSCOMITRELLA-PATENS; MITOTIC SPINDLES; MAMMALIAN-CELLS; BUDDING YEAST; PLANT-CELLS; ORGANIZATION;
Keywords:
APICAL CELL; CENTRIN; CENTROSOME; MICTROTUBULE; MITOSIS; PHAEOPHYCEAE; SPHACELARIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
D. Karyophyllis et al., "CENTROSOME AND MICROTUBULE DYNAMICS IN APICAL CELLS OF SPHACELARIA-RIGIDULA (PHAEOPHYCEAE) TREATED WITH NOCODAZOLE", Protoplasma, 199(3-4), 1997, pp. 161-172

Abstract

Treatment of young thalli of Sphacelaria rigidula with 0.04 mu g of nocodazole (Nz) per mi for up to 36 h affects microtubules (Mts) only slightly, but blocks a large number of mitotic cells in metaphase, without disruption of the metaphase plate. Higher concentrations of Nz (0.1 mu g/ml) depolymerize interphase Mts. Only a few perinuclear and some short Mts resist and remain associated with the centrosomes. Fragmented Mts or groups of Mts sometimes remain in the apical dome. After treatment with 0.1 mu g of Nz per mi, prometaphase cells are blocked at metaphase, while post-metaphase cells become binuclear, due to the failure of cytokinesis. With anticentrin immunofluorescence, a positive centrin signal is always observed in the centrosome area. Centrosome duplication is not affected by Nz, but separation is disturbed. After recovering for 2-4 h, most of the blocked metaphases proceed normally. In such cells duplicated centrosomes are seen in different stages of separation. In some cells independent aster-like microtubule configurations appear in the apical dome, occasionally displaying centrin at their centre. During recovery various configurations of bimitosis or multipolar mitosis were found. The multipolar spindles may share common centrosomes. Up to four centrosomes may accompany each nucleus. In some 24 h treated cells, as well as in cells recovering for 2 h, the centrin-positive structure is rod-like, extending in opposite directions fromthe usual position to the poles. Electron microscopical examination of thin sections revealed that the growth pattern of the apical cells is disrupted after Nz treatment. The observations show that: (a) the Mtcytoskeleton is involved in maintaining the polarity and growth pattern of apical cells, (b) mitosis is blocked by low concentrations of Nzwithout significant depolymerization of Mts, (c) the centrosome cycleis independent of the nuclear cycle, (d) centrosome separation and differentiation are disturbed by Nz treatment, (e) during recovery from Nz treatment, centrosomal material that may have separated from the centrosomes, as well as Mt fragments that resisted depolymerization, mayoperate as MI nucleation centres.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 16:06:41