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Titolo:
ACTIN FILAMENT BUNDLES IN CORTICAL FIBER CELLS OF THE RAT LENS
Autore:
LO WK; SHAW AP; WEN XJ;
Indirizzi:
MOREHOUSE SCH MED,DEPT ANAT,720 WESTVIEW DR SW ATLANTA GA 30310
Titolo Testata:
Experimental Eye Research
fascicolo: 5, volume: 65, anno: 1997,
pagine: 691 - 701
SICI:
0014-4835(1997)65:5<691:AFBICF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOSKELETAL PROTEIN; STRUCTURAL PROTEIN; BINDING PROTEINS; EPITHELIAL-CELLS; SQUID AXOPLASM; DIFFERENTIATION; MECHANISMS; FILENSIN; ACCOMMODATION; LOCALIZATION;
Keywords:
ACTIN BUNDLES; LENS FIBER CELLS; ADHERENS JUNCTIONS; INTERMEDIATE FILAMENTS; ALPHA-ACTININ; RATS; ELECTRON MICROSCOPY; IMMUNOCYTOCHEMISTRY; IMMUNOBLOTTING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
W.K. Lo et al., "ACTIN FILAMENT BUNDLES IN CORTICAL FIBER CELLS OF THE RAT LENS", Experimental Eye Research, 65(5), 1997, pp. 691-701

Abstract

The distribution and organization of actin filament bundles were studied in cortical fiber cells of rat lenses at various ages (3 days to 2.5 months old), using thin-section electron microscopy, immunocytochemistry and immunoblotting. Electron microscopy showed that actin bundles were regularly found along cortical fiber cell membranes of the lensat all ages studied. The actin bundles were commonly arranged in three distinct units, one bundle in each fiber cell, located at the intersections where three hexagonal fiber cells meet as seen in cross sections. These actin bundles were approximately 150 nm in diameter and werecomposed of 7-nm small filaments. They were aligned parallel to the long axis of fiber cells as judged by both cross and longitudinal sections. The outside border of each bundle was always surrounded by a zoneof 10-nm intermediate filaments which have the same orientation as that of the actin bundles. In longitudinal sections, elongated actin bundles were always parallel to the cell membranes. A number of individual actin bundles sometimes were found to form a chain with periodic short intervals. In addition, actin bundles were frequently associated with adherens junctions near the intersections and other regions of fiber cell membranes. By immunoelectron microscopy, we demonstrated that these filament bundles indeed contained actins. By rhodamine-phalloidinlabelling, we found that labeled actin bundles appeared as large, distinct dots at the corners of hexagonal fiber cells in all ages studied. In addition, nonbundle F-actins were labeled preferentially along the cell membranes of the short sides of hexagonal fiber cells. This resulted in a unique zigzag pattern of actin labeling commonly seen in the cortical fiber cells of a mature rat lens. Finally, we showed that alpha-actinin was associated with the actin bundles in the fiber cells by immunofluorescent double labeling and immunoblotting. It is suggested that this unique arrangement of actin bundles in fiber cells may provide a stabilizing structure for forming a sharp angle at each cornerof fiber cells, thereby the hexagonal shape of the cells can be maintained. (C) 1997 Academic Press Limited.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:12:16