Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
E1A 12S AND 13S OF THE TRANSFORMATION-DEFECTIVE ADENOVIRUS-TYPE-12 STRAIN CS-1 INACTIVATE PROTEINS OF THE RB FAMILY, PERMITTING TRANSACTIVATION OF THE E2F-DEPENDENT PROMOTER
Autore:
PUTZER BM; RUMPF H; REGA S; BROCKMANN D; ESCHE H;
Indirizzi:
UNIV ESSEN GESAMTHSCH,SCH MED,INST MOL BIOL CANC RES,HUFELANDSTR 55 D-45122 ESSEN GERMANY
Titolo Testata:
Journal of virology
fascicolo: 12, volume: 71, anno: 1997,
pagine: 9538 - 9548
SICI:
0022-538X(1997)71:12<9538:E1A1OT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR E2F; EARLY-REGION 1A; LARGE-T-ANTIGEN; RETINOBLASTOMA PROTEIN; CELL-CYCLE; GENE-EXPRESSION; MESSENGER-RNA; HOST RANGE; VERO CELLS; 2 REGIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
66
Recensione:
Indirizzi per estratti:
Citazione:
B.M. Putzer et al., "E1A 12S AND 13S OF THE TRANSFORMATION-DEFECTIVE ADENOVIRUS-TYPE-12 STRAIN CS-1 INACTIVATE PROTEINS OF THE RB FAMILY, PERMITTING TRANSACTIVATION OF THE E2F-DEPENDENT PROMOTER", Journal of virology, 71(12), 1997, pp. 9538-9548

Abstract

The transformation-defective Vero cell host range mutant CS-1 of the highly oncogenic adenovirus type 12 (Ad12) (Ad12-CS-1) has a 69-bp deletion in the early region 1A (E1A) gene that removes the carboxy-terminal half of conserved region 2 and the amino-terminal half of the Ad12-specific so-called spacer that seems to play a pivotal role in the oncogenicity of the virus. Despite its deficiency in immortalizing and transforming primary rodent cells, we found that the E1A 13S protein ofAd12-CS-1 retains the ability to bind p105-RB, p107, and p130 in nuclear extract binding assays with glutathione S-transferase-E1A fusion proteins and Western blot analysis. Like wild-type E1A, the mutant protein was able to dissociate E2F from retinoblastoma-related protein-containing complexes, as judged from gel shift experiments with purified 12S and 13S proteins from transfection experiments with an E1A expression vector or from infection with the respective virus. Moreover, in transient expression assays, the 12S and 13S products of wild-type Ad12and Ad12-CS-1 were shown to transactivate the Ad12 E1A promoter containing E2F-1 and E2F-5-motifs, respectively, in a comparable manner. The same results were obtained from transfection assays with the E2F motif-dependent E2 promoter of adenovirus type 5 or the human dihydrofolate reductase promoter. These data suggest that efficient infection by Ad12 and the correlated virus-induced reprogramming of the infected cells, including the induction of cell cycle-relevant mechanisms (e.g. E2F activation), can be uncoupled from the transformation properties ofthe virus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 00:48:26