Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE 3'-UNTRANSLATED REGION OF THE B19 PARVOVIRUS CAPSID PROTEIN MESSENGER-RNAS INHIBITS ITS OWN MESSENGER-RNA TRANSLATION IN NONPERMISSIVE CELLS
Autore:
PALLIER C; GRECO A; LEJUNTER J; SAIB A; VASSIAS I; MORINET F;
Indirizzi:
HOP ST LOUIS,MICROBIOL SERV,VIROL UNIT,1 AVE CLAUDE VELLEFAUX F-75475PARIS 10 FRANCE HOP ST LOUIS,MICROBIOL SERV,VIROL UNIT F-75475 PARIS 10 FRANCE HOP ST LOUIS,CNRS,UPR 9051 F-75475 PARIS 10 FRANCE FAC MED LYON RTH LAENNEC,CNRS,UMR 5537 F-69372 LYON 08 FRANCE
Titolo Testata:
Journal of virology
fascicolo: 12, volume: 71, anno: 1997,
pagine: 9482 - 9489
SICI:
0022-538X(1997)71:12<9482:T3ROTB>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; NONSTRUCTURAL PROTEIN; VIRUS TYPE-1; GENE-EXPRESSION; MINUTE VIRUS; IDENTIFICATION; SEQUENCE; INVITRO; REPLICATION; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
C. Pallier et al., "THE 3'-UNTRANSLATED REGION OF THE B19 PARVOVIRUS CAPSID PROTEIN MESSENGER-RNAS INHIBITS ITS OWN MESSENGER-RNA TRANSLATION IN NONPERMISSIVE CELLS", Journal of virology, 71(12), 1997, pp. 9482-9489

Abstract

Although parvoviruses are found throughout the animal kingdom, only the human pathogenic B19 virus has so far been shown to possess a limited host range, with erythroid progenitor cells as the main target cells supporting B19 propagation. The underlying mechanism of such erythroid tropism is still unexplained, Synthesis of the NS1 nonstructural protein occurs in permissive and nonpermissive cells, such as megakaryocytes, whereas synthesis of the VP1 and VP2 capsid proteins seems to berestricted to burst-forming units arid CFU of erythroid cells. In nonpermissive cells, the NS1 protein is overexpressed and the NS1 RNAs are the predominant RNA species, However, the VP1 and VP2 proteins are not detectable, although the corresponding mRNAs are synthesized, Sinceall transcripts have part of the 5' untranslated region (5' UTR) in common but distinct 3' UTRs characterizing the nonstructural-and structural-protein mRNAs, we investigated, in transient transfection assays,the possible involvement of the 3' UTR of the capsid protein mRNAs inVP1 and VP2 protein synthesis in nonpermissive Cos cells, The resultsshowed that (i) the 3' UTR of mRNAs coding for the capsid proteins repressed VP1 and VP2 protein synthesis, (ii) the 3' UTR did not affect nuclear expert or mRNA stability, and (iii) mRNAs bearing the 3' UTR of the capsid protein mRNAs did not associate with ribosomes at all, Taken together, these results indicate that in nonpermissive cells, the 3' UTR of the capsid protein mRNAs represses capsid protein synthesis at the translational level by inhibiting ribosome loading.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:56:16