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Titolo:
ROUS-SARCOMA VIRUS DIRECT REPEAT CIS-ELEMENTS EXERT EFFECTS AT SEVERAL POINTS IN THE VIRUS LIFE-CYCLE
Autore:
SIMPSON SB; ZHANG L; CRAVEN RC; STOLTZFUS CM;
Indirizzi:
UNIV IOWA,DEPT MICROBIOL IOWA CITY IA 52242 UNIV IOWA,DEPT MICROBIOL IOWA CITY IA 52242 PENN STATE UNIV,COLL MED,DEPT MICROBIOL & IMMUNOL HERSHEY PA 17033
Titolo Testata:
Journal of virology
fascicolo: 12, volume: 71, anno: 1997,
pagine: 9150 - 9156
SICI:
0022-538X(1997)71:12<9150:RVDRCE>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRAL MESSENGER-RNA; 3' SPLICE-SITE; MAMMALIAN-CELLS; NUCLEAR EXPORT; SRC GENE; UNSPLICED RNA; REV PROTEIN; REPLICATION; TYPE-1; FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
S.B. Simpson et al., "ROUS-SARCOMA VIRUS DIRECT REPEAT CIS-ELEMENTS EXERT EFFECTS AT SEVERAL POINTS IN THE VIRUS LIFE-CYCLE", Journal of virology, 71(12), 1997, pp. 9150-9156

Abstract

Two similar to 135-nucleotide (nt) direct repeats flank the Rous sarcoma virus (RSV) oncogene src and are composed of two smaller repeats, drl (similar to 100 nt) and dr2 (similar to 36 nt), These sequences have been reported to contain cis-acting signals necessary for RNA packaging and elements that allow cytoplasmic accumulation of unspliced RNA(cytoplasmic transport elements). In this report, we show that avian fibroblasts infected with the Prague A strain of RSV with precise deletions of both drl elements express src and are transformed by this mutant virus but production of virus particles is very low and virus spread throughout the culture requires several weeks. We show that the replication defect is due to complex effects on viral RNA transport, viral RNA half-life, and virus particle assembly. The dr1 elements may contain binding sites for a permissive cell-specific factor(s) that facilitates efficient nuclear-cytoplasmic transport, RNA stability, and cytoplasmic utilization of unspliced viral RNA, The implications of theseresults for understanding the defects of nonpermissive virus Infections in mammalian cells are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 10:26:23