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Titolo:
INDUCTION OF SIMILAR FEATURES OF APOPTOSIS IN HUMAN AND BOVINE VASCULAR ENDOTHELIAL-CELLS TREATED BY 7-KETOCHOLESTEROL
Autore:
LIZARD G; MOISANT M; CORDELET C; MONIER S; GAMBERT P; LAGROST L;
Indirizzi:
CHRU,HOP BOCAGE,INSERM CJF 93 10,LAB BIOCHIM MED,BP 1542 F-21034 DIJON FRANCE FAC MED,INSERM CJF 93 10,LAB BIOCHIM LIPOPROT F-21033 DIJON FRANCE FAC MED,HISTOL LAB F-21033 DIJON FRANCE INRA,UNITE NUTR LIPID F-21000 DIJON FRANCE
Titolo Testata:
Journal of pathology
fascicolo: 3, volume: 183, anno: 1997,
pagine: 330 - 338
SICI:
0022-3417(1997)183:3<330:IOSFOA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN MONOCYTE-MACROPHAGES; SMOOTH-MUSCLE CELLS; OXIDIZED LDL; CHOLESTEROL OXIDES; CYTOTOXICITY; OXYSTEROLS; OXIDATION; DEATH; ATHEROSCLEROSIS;
Keywords:
ENDOTHELIAL CELLS; 7-KETOCHOLESTEROL; APOPTOSIS; DNA FRAGMENTATION; ULTRASTRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
G. Lizard et al., "INDUCTION OF SIMILAR FEATURES OF APOPTOSIS IN HUMAN AND BOVINE VASCULAR ENDOTHELIAL-CELLS TREATED BY 7-KETOCHOLESTEROL", Journal of pathology, 183(3), 1997, pp. 330-338

Abstract

Cholesterol oxides have numerous cytotoxic effects and those oxidizedin the C7 position hare been shown to induce apoptosis in bovine aortic endothelial cells (BAEC). The aim of the present study was to determine whether apoptosis also occurs in human vascular endothelial cells(HUVEC) treated with 7-ketocholesterol. To this end, cultured BAEC and HUVEC were incubated for 48 h with 7-ketocholesterol (concentration range 5-80 mu g/ml) and the characteristics of cell death mere assessed by various methods: counting of adherent and non-adherent cells; analysis of DNA fragmentation pattern; and morphological study by light, fluorescence, and electron microscopy. The 7-ketocholesterol treatmentwas accompanied by a decrease in the number of adherent cells and an increase in the number of non-adherent cells. Apoptotic cells, recognized by fragmented and/or condensed nuclei after staining with Hoechst 33342 or Giemsa, were mainly detected among non-adherent cells, and agarose gel electrophoresis revealed a typical internucleosomal DNA fragmentation among 7-ketocholesterol-treated cells. The DNA fragmentationmas no longer detected a hen HUVEC and BAEC were simultaneously incubated with 0.5 mmol/l zinc chloride, which is known to inhibit Ca2+/Mg2-dependent endonucleases. Finally, the ultrastructural abnormalities observed by electron microscopy in both 7-ketocholesterol-treated HUVEC and BAEC were remarkably similar and were mainly characterized by condensed chromatin, altered mitochondria, disturbed organization of thecytoskeleton, and vacuoles containing myelin figures and/or cell debris; apoptotic bodies mere also frequently detected. It is concluded that 7-ketocholesterol constitutes a potent inducer of apoptosis in endothelial vascular cells of both bovine and human origin, suggesting that cholesterol oxides may be involved in the early steps of the atherosclerotic process in humans. (C) 1997 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 08:57:05