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Titolo:
ESTRADIOL, A NEW HAPTEN FOR DETECTING NUCLEIC-ACID SEQUENCES BY FISH
Autore:
VANDECORPUT MPC; DIRKS RW; WIEGANT WW; WIEGANT J; MUHLEGGER K; RAAP AK;
Indirizzi:
LEIDEN STATE UNIV,DEPT MOL CELL BIOL,LAB CYTOCHEM & CYTOMETRY,WASSENAARSEWEG 72 NL-2333 AL LEIDEN NETHERLANDS LEIDEN STATE UNIV,DEPT MOL CELL BIOL,LAB CYTOCHEM & CYTOMETRY NL-2333AL LEIDEN NETHERLANDS BOEHRINGER MANNHEIM GMBH,WERK PENZBERG D-82372 PENZBERG GERMANY
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 4-5, volume: 108, anno: 1997,
pagine: 359 - 364
SICI:
0948-6143(1997)108:4-5<359:EANHFD>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE INSITU HYBRIDIZATION; DNA PROBES; RNA; MULTIPLE; CHROMOSOMES; SATELLITE; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
M.P.C. Vandecorput et al., "ESTRADIOL, A NEW HAPTEN FOR DETECTING NUCLEIC-ACID SEQUENCES BY FISH", HISTOCHEM C, 108(4-5), 1997, pp. 359-364

Abstract

Oestradiol has been conjugated to allylamine-dUTP with an 11-atom spacer to allow enzymatic incorporation of the label into DNA sequences. In a comparative DNA and mRNA FISH study we have used DNA probes that were either labelled with digoxigenin, biotin or oestradiol. Results show that oestradiol-labelled probes can detect DNA and RNA sequences in FISH equally well as digoxigenin-and biotin-labelled probes. Further, no crossreactivity between the various hapten-specific antibodies and the three haptens were observed. Binding of the rabbit anti-oestradiol antibody to endogenous oestrogen in various tissues was not observed under the conditions tested. In view of the increasing demands for multi-colour DNA and mRNA FISH applications, oestradiol is a welcome addition to the collection of haptens employed in FISH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 14:01:10