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Titolo:
DISTRIBUTION AND MOBILITY OF THE TETRACYCLINE RESISTANCE DETERMINANT TETQ
Autore:
LENG ZT; RILEY DE; BERGER RE; KRIEGER JN; ROBERTS MC;
Indirizzi:
UNIV WASHINGTON,SCH PUBL HLTH & COMMUNITY MED,DEPT PATHOBIOL,BOX 357238 SEATTLE WA 98195 UNIV WASHINGTON,SCH PUBL HLTH & COMMUNITY MED,DEPT PATHOBIOL SEATTLE WA 98195 UNIV WASHINGTON,DEPT UROL SEATTLE WA 98195
Titolo Testata:
Journal of antimicrobial chemotherapy
fascicolo: 4, volume: 40, anno: 1997,
pagine: 551 - 559
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; POLYMERASE CHAIN-REACTION; GENETIC-BASIS; BACTERIA; PROTECTION; PREVOTELLA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
Z.T. Leng et al., "DISTRIBUTION AND MOBILITY OF THE TETRACYCLINE RESISTANCE DETERMINANT TETQ", Journal of antimicrobial chemotherapy, 40(4), 1997, pp. 551-559

Abstract

We tested 34 American Type Culture Collection (ATCC) and 168 clinicalbacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetQ gene using a polymerase chain reaction (PCR) assay and DNA-DNA hybridization. The identities of PCR products were confirmed by Southern blot hybridization of whole-cell DNA. Eleven of the ATCC strains were positive for tetQ, including five Bacteroides spp., five Prevotella spp. and a single isolate of Mitsuokella multiacidus. Twenty-eight (29%) of the 95 clinical Gram-negative isolates carried the tetQ gene, whileeight (11%) of the 73 clinical Gram-positive isolates carried the tetQ gene. This is the first description of tetQ in Gram-positive species. All isolates except one Peptostreptococcus sp. carried tetQ integrated into the chromosome. The tetQ gene could be transferred from Prevotella bivia, Bacteroides ovatus, Bacteroides fragilis, Bacteroides vulgatus and Bacteroides distasonis into an Enterococcus faecalis recipient at frequencies of 10(-7)-10(-9) per recipient. In contrast, tetQ failed to transfer from two isolates of Prevotella intermedia, two isolates of Porphyromonas gingivalis, one isolate of Mobiluncus curtisii andone isolate of Peptostreptococcus sp. The latter two are Gram-positive species. The PCR assay was used to screen 198 proteinase K-treated biopsies of prostate, periprostate and bladder from 84 men with prostatitis. Thirty-four (40%) of the patients had one or more positive samples, suggesting that the PCR assay could be of value in screening patient material directly for the presence of bacteria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 19:05:18