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Titolo:
TOWARDS THE PHYSICAL MAP OF THE TRYPANOSOMA-CRUZI NUCLEAR GENOME - CONSTRUCTION OF YAC AND BAC LIBRARIES OF THE REFERENCE CLONE TRYPANOSOMA-CRUZI CL-BRENER
Autore:
FERRARI I; LORENZI H; SANTOS MR; BRANDARIZ S; REQUENA JM; SCHIJMAN A; VAZQUEZ M; DASILVEIRA JF; BENDOV C; MEDRANO C; GHIO S; BERGAMI PL; CANO I; ZINGALES B; URMENYI TP; RONDINELLI E; GONZALEZ A; CORTES A; LOPEZ MC; THOMAS MC; ALONSO C; RAMIREZ JL; CHIURRILLO MA; ALDAO RR; BRANDAO A; DEGRAVE W; PERROT V; SAUMIER M; BILLAUT A; COHEN D; LEPASLIER D; LEVIN MJ;
Indirizzi:
UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,CONICET,INGEBI,INST INVEST INGN GENET & BIOL MOL RA-1428 BUENOS AIRES DF ARGENTINA UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,CONICET,INGEBI,INST INVEST INGN GENET & BIOL MOL RA-1428 BUENOS AIRES DF ARGENTINA ESCOLA PAULISTA MED SAO PAULO SP BRAZIL CBM MADRID SPAIN UNIV SAO PAULO BR-05508 SAO PAULO SP BRAZIL UNIV FED RIO DE JANEIRO,CCS BR-21941 RIO JANEIRO RJ BRAZIL IPB GRANADA SPAIN CENT UNIV VENEZUELA CARACAS VENEZUELA USB CARACAS VENEZUELA INST OSWALDO CRUZ RIO JANEIRO RJ BRAZIL CTR ETUD POLYMORPHISME HUMAIN F-75010 PARIS FRANCE
Titolo Testata:
Memorias do Instituto Oswaldo Cruz
fascicolo: 6, volume: 92, anno: 1997,
pagine: 843 - 852
SICI:
0074-0276(1997)92:6<843:TTPMOT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHAGAS-DISEASE; PLASMODIUM-FALCIPARUM; DNA; CHROMOSOME; IDENTIFICATION; VARIABILITY; KARYOTYPE; FRAGMENTS; FAMILY; GENES;
Keywords:
TRYPANOSOMA CRUZI; GENOME PROJECT; PHYSICAL MAP; YAC LIBRARY; BAC LIBRARY; SEQUENCE TAGGED SITES (STS); EXPRESSED SEQUENCE TAGS (EST);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
I. Ferrari et al., "TOWARDS THE PHYSICAL MAP OF THE TRYPANOSOMA-CRUZI NUCLEAR GENOME - CONSTRUCTION OF YAC AND BAC LIBRARIES OF THE REFERENCE CLONE TRYPANOSOMA-CRUZI CL-BRENER", Memorias do Instituto Oswaldo Cruz, 92(6), 1997, pp. 843-852

Abstract

Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have tn capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomescan be defined and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries ill bacterial artificial chromosomes (BACs) have been constructed BACI and BACII. Both libraries represent about three genome equivalents. A third BAClibrary (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be consideredas a common resource for research in Chagas disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:57:21