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Titolo:
ESSENTIAL DIFFERENCES IN CHOLESTERYL ESTER METABOLISM BETWEEN HUMAN MONOCYTE-DERIVED AND J774 MACROPHAGES - EVIDENCE AGAINST THE PRESENCE OF HORMONE-SENSITIVE LIPASE IN HUMAN MACROPHAGES
Autore:
CONTRERAS JA; LASUNCION MA;
Indirizzi:
HOSP RAMON Y CAJAL,SERV BIOQUIM INVEST,UNIDAD DISLIPEMIAS,CTRA COLMENAR,KM 9 E-28034 MADRID SPAIN HOSP RAMON Y CAJAL,SERV BIOQUIM INVEST,UNIDAD DISLIPEMIAS E-28034 MADRID SPAIN UNIV ALCALA DE HENARES MADRID SPAIN
Titolo Testata:
Arteriosclerosis and thrombosis
fascicolo: 3, volume: 14, anno: 1994,
pagine: 443 - 452
SICI:
1049-8834(1994)14:3<443:EDICEM>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT PROTEIN-KINASE; LOW-DENSITY-LIPOPROTEIN; CYCLIC ADENOSINE-MONOPHOSPHATE; HUMAN ATHEROSCLEROTIC LESIONS; FATTY STREAK FORMATION; SMOOTH-MUSCLE CELLS; FOAM CELLS; ACYL-COA; PHOSPHORYLATION-DEPHOSPHORYLATION; IMMUNOCYTOCHEMICAL ANALYSIS;
Keywords:
HUMAN MONOCYTE-DERIVED MACROPHAGES; NEUTRAL CHOLESTERYL ESTER HYDROLASE; HORMONE-SENSITIVE LIPASE; CAMP; J774 MACROPHAGES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Contreras e M.A. Lasuncion, "ESSENTIAL DIFFERENCES IN CHOLESTERYL ESTER METABOLISM BETWEEN HUMAN MONOCYTE-DERIVED AND J774 MACROPHAGES - EVIDENCE AGAINST THE PRESENCE OF HORMONE-SENSITIVE LIPASE IN HUMAN MACROPHAGES", Arteriosclerosis and thrombosis, 14(3), 1994, pp. 443-452

Abstract

Cholesteryl ester-laden macrophages are the hallmark of the fatty streaks that precede arteriosclerotic plaques in humans and experimental animals. This article studies several aspects of cytoplasmic cholesteryl ester metabolism in cultured human monocyte-derived macrophages. Adenosine 3',5'-cyclic monophosphate (cAMP) consistently inhibited cholesteryl ester mobilization from cells that had been loaded with cholesteryl esters by preincubation with acetylated low-density lipoprotein. This effect was observed in both the absence and presence of extracellular cholesterol accepters as well as with acyl coenzyme A:cholesterolacyltransferase inhibitors. In contrast, dibutyryl cAMP activated cholesteryl ester hydrolysis in J774 macrophages. Since hormone-sensitivelipase is thought to be responsible for the neutral cholesteryl esterhydrolytic activity in several cell types, we looked for the presenceof its mRNA in our macrophages by means of reverse transcription coupled to the polymerase chain reaction technique. Hormone-sensitive lipase mRNA was detected in J774 macrophages but not in human monocytes orin human monocyte-derived macrophages. These results demonstrated great differences in cholesteryl ester metabolism between macrophages of different origin. While hormone-sensitive lipase may be responsible for neutral cholesteryl ester hydrolytic activity in J774 macrophages, in human monocyte-derived macrophages it is not; thus, a different and as yet unidentified enzyme must be present. (Arterioscler Thromb. 1994;14:443-452.)

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/02/20 alle ore 02:29:19