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Titolo: A CYTOSOLIC SPERM PROTEIN FACTOR MOBILIZES CA2-MOLECULAR-WEIGHT MESSENGERS( FROM INTRACELLULAR STORES BY ACTIVATING MULTIPLE CA2+ RELEASE MECHANISMS INDEPENDENTLY OF LOW)
Autore: GALIONE A; JONES KT; LAI FA; SWANN K;
- Indirizzi:
- UNIV LONDON UNIV COLL,DEPT ANAT & DEV BIOL,GOWER ST LONDON WC1E 6BT ENGLAND UNIV OXFORD,DEPT PHARMACOL OXFORD OX1 3QT ENGLAND NATL INST MED RES,MRC LONDON NW7 1AA ENGLAND
- Titolo Testata:
- The Journal of biological chemistry
fascicolo: 46,
volume: 272,
anno: 1997,
pagine: 28901 - 28905
- SICI:
- 0021-9258(1997)272:46<28901:ACSPFM>2.0.ZU;2-F
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- CYCLIC ADP-RIBOSE; SEA-URCHIN EGGS; INOSITOL TRISPHOSPHATE; CALCIUM OSCILLATIONS; MAMMALIAN EGGS; FERTILIZATION; WAVES; THAPSIGARGIN; RECEPTORS; RYANODINE;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Citazioni:
- 31
- Recensione:
- Indirizzi per estratti:
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- Citazione:
- A. Galione et al., "A CYTOSOLIC SPERM PROTEIN FACTOR MOBILIZES CA2-MOLECULAR-WEIGHT MESSENGERS( FROM INTRACELLULAR STORES BY ACTIVATING MULTIPLE CA2+ RELEASE MECHANISMS INDEPENDENTLY OF LOW)", The Journal of biological chemistry, 272(46), 1997, pp. 28901-28905
Abstract
Ca2+ oscillations can be induced in mammalian eggs and somatic cells by microinjection of a cytosolic sperm protein factor. The nature of the sperm factor-induced Ca2+ signaling was investigated by adding sperm protein extracts to homogenates of sea urchin eggs, which contain multiple classes of Ca2+ release mechanisms. We show that the sperm factor mobilizes Ca2+ from nonmitochondrial Ca2+ stores in egg homogenatesafter a distinct latency. This latency is abolished by preincubation of sperm extracts with egg cytosol. The preincubation step is highly temperature-dependent and generates a high molecular weight, protein-based Ca2+-releasing agent that can also mobilize Ca2+ from purified eggmicrosomes. This Ca2+ release appears to be mediated via both inositol 1,4,5-trisphosphate and ryanodine receptors, since homologous desensitization of these two release mechanisms by their respective agonistsinhibits further release by the sperm factor. However, sperm factor-induced Ca2+ release by these channels is independent of inositol 1,4,5-trisphosphate or cADPR since antagonists of either of these two messengers did not block the Ca2+ release effected by the sperm factor. Thesperm protein factor may cause Ca2+ release via an enzymatic step that generates a protein-based Ca2+-releasing agent.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:54:02