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Titolo:
CASEIN KINASE-II REGULATION OF YEAST TFIIIB IS MEDIATED BY THE TATA-BINDING PROTEIN
Autore:
GHAVIDEL A; SCHULTZ MC;
Indirizzi:
UNIV ALBERTA,DEPT BIOCHEM EDMONTON AB T6G 2H7 CANADA UNIV ALBERTA,DEPT BIOCHEM EDMONTON AB T6G 2H7 CANADA
Titolo Testata:
Genes & development
fascicolo: 21, volume: 11, anno: 1997,
pagine: 2780 - 2789
SICI:
0890-9369(1997)11:21<2780:CKROYT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-III; SACCHAROMYCES-CEREVISIAE; MITOTIC REPRESSION; CRYSTAL-STRUCTURE; TRANSCRIPTION; GENE; DNA; PROMOTERS; SUBUNIT; INVITRO;
Keywords:
TRANSCRIPTION; RNA POLYMERASE III; TFIIIB; TBP; CASEIN KINASE II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
A. Ghavidel e M.C. Schultz, "CASEIN KINASE-II REGULATION OF YEAST TFIIIB IS MEDIATED BY THE TATA-BINDING PROTEIN", Genes & development, 11(21), 1997, pp. 2780-2789

Abstract

The highly conserved protein kinase casein kinase II (CKII) is required for efficient Pol III transcription of the tRNA and 5S rRNA genes in Saccharomyces cerevisiae. Using purified factors from wild-type cells to complement transcription extracts from a conditional lethal mutant of CKII we show that TFIIIB is the CKII-responsive component of the Pol III transcription machinery. Dephosphorylation of TFIIIB eliminated its ability to complement CKII-depleted extract, and a single TFIIIBsubunit, the TATE-binding protein (TBP), is a preferred substrate of CKII in vitro. Recombinant TBP purified from Escherichia coli is phosphorylated efficiently by CKII and, in the presence of a limiting amount of CKII, is able to substantially rescue transcription in CKII-deficient extract. Our results establish that TBP is a key component of thepathway linking CKII activity and Pol III transcription and suggest that TBP is the target of a CKII-mediated regulatory mechanism that canmodulate Pol III transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:30:59