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Titolo:
EFFECTS OF ADRENERGIC AND MUSCARINIC AGONIST STIMULATION ON IP3 AND CYCLIC-NUCLEOTIDE LEVELS IN THE PRESSURE OVERLOADED RAT-HEART
Autore:
MARTINUSSEN HJ; WALDENSTROM A; RONQUIST G;
Indirizzi:
UNIV UPPSALA HOSP,DEPT CLIN CHEM S-75185 UPPSALA SWEDEN UNIV UPPSALA HOSP,DEPT CLIN CHEM S-75185 UPPSALA SWEDEN UNIV UPPSALA HOSP,DEPT ANAESTHESIOL S-75185 UPPSALA SWEDEN UNIV UPPSALA HOSP,DEPT CARDIOL S-75185 UPPSALA SWEDEN
Titolo Testata:
Scandinavian journal of clinical & laboratory investigation
fascicolo: 7, volume: 57, anno: 1997,
pagine: 587 - 594
SICI:
0036-5513(1997)57:7<587:EOAAMA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
VENTRICULAR PAPILLARY-MUSCLES; CARDIAC-HYPERTROPHY; INOSITOL TRISPHOSPHATE; METABOLISM;
Keywords:
CARBACHOL; CYCLIC NUCLEOTIDES; INOSITOL TRISPHOSPHATE; MUSCARINIC-RECEPTOR; MYOCARDIUM; NORADRENALINE; PRESSURE OVERLOADED; RAT; TRISPHOSPHATE; BETA-RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
H.J. Martinussen et al., "EFFECTS OF ADRENERGIC AND MUSCARINIC AGONIST STIMULATION ON IP3 AND CYCLIC-NUCLEOTIDE LEVELS IN THE PRESSURE OVERLOADED RAT-HEART", Scandinavian journal of clinical & laboratory investigation, 57(7), 1997, pp. 587-594

Abstract

In this study, the dynamic interrelationships between myocardial functional state and changes in the second messenger content in pressure-overloaded hypertrophied hearts were investigated. Forty-three rat hearts were used after partial clamping of the abdominal aorta. The isolated hearts were perfused with Krebs-Henseleit buffer and allocated to perfusion for 20 s or 40 min as controls (n=12); or with noradrenaline (10(-6) moll(-1), n=11); carbachol (3x10(-7) mol l(-1), n = 9); or noradrenaline plus carbachol (10(-6) mol l(-1) + 3 x 10(-7) mol l(-1), respectively, n=11).(max)dP/dt increased more than 2-fold already after 20 s on noradrenaline stimulation, followed by a significant increase in cAMP. After 40 min,(max)dP/dt was lower than the maximal value, although higher than controls. cAMP was also decreased, but still significantly higher than controls. Perfusion with noradrenaline plus carbachol produced the same changes in (max)dP/dt as those seen after noradrenaline stimulation alone, but failed to increase cAMP content after both 20s and 40 min. The inositol trisphosphate (IP3) content was increased 40 min of control perfusion (p<0.05). Noradrenaline and carbachol,separately, produced an increase in IF3 content already after 20 s (p<0.05). The combination of noradrenaline plus carbachol also produced an increase of IP3 (p<0.05; compared to controls), but to a lesser extent when compared either to noradrenaline or carbachol (p<0.05). After40 min of perfusion, IP3 was in the same range regardless of added agonist(s) and still slightly above control level (p<0.05). The early increase in maxdP/dt induced by noradrenaline or the combination of noradrenaline plus carbachol was not paralleled by a decrease in ATP content. This was also the case upon addition of carbachol alone. However, after 40 min of agonistic perfusion, ATP levels were substantially decreased. In conclusion, myocardial IP3 content in pressure-overloaded hypertrophied hearts was not different from that of sham-operated hearts. After agonistic stimulation, an early increase in IP3 formation wasseen. Attenuation of the IF, response by combined stimulation with noradrenaline and carbachol was initially present in pressure-overloadedhypertrophied hearts. After 40 min no attenuation was found for either IP3 or for cAMP content, suggestive of induction of a desensitization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 15:03:34