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Titolo:
PROSTAGLANDIN-D SYNTHASE IN HUMAN MEGAKARYOBLASTIC CELLS
Autore:
MAHMUD I; UEDA N; YAMAGUCHI H; YAMASHITA R; YAMAMOTO S; KANAOKA Y; URADE Y; HAYAISHI O;
Indirizzi:
UNIV TOKUSHIMA,SCH MED,DEPT BIOCHEM,KURAMOTO CHO TOKUSHIMA 770 JAPAN UNIV TOKUSHIMA,SCH MED,DEPT BIOCHEM TOKUSHIMA 770 JAPAN UNIV TOKUSHIMA,INST ENZYME RES TOKUSHIMA 770 JAPAN OSAKA BIOSCI INST,DEPT MOL BEHAV BIOL SUITA OSAKA 565 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 45, volume: 272, anno: 1997,
pagine: 28263 - 28266
SICI:
0021-9258(1997)272:45<28263:PSIHMC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
D SYNTHETASE; CDNA CLONING; D-ISOMERASE; BETA-TRACE; PURIFICATION; LINE; IDENTIFICATION; PROTEIN; CMK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
I. Mahmud et al., "PROSTAGLANDIN-D SYNTHASE IN HUMAN MEGAKARYOBLASTIC CELLS", The Journal of biological chemistry, 272(45), 1997, pp. 28263-28266

Abstract

The cytosol fraction of human platelets did not convert prostaglandin(PG) H-2 to PGD(2). However, a homogenate of human megakaryoblastic CMK cells (precursor cells of platelets) produced PGD(2) from PGH(2). The PGD synthase activity was localized in the cytosol of CMK cells, and absolutely required glutathione. The catalytic properties and Western and Northern blottings indicated that the enzyme was PGD synthase ofthe hematopoietic type rather than the lipocalin type, When CMK cellswere differentiated to megakaryocytes with phorbol ester along with induction of cyclooxygenase-l, the PGD synthase activity increased about 2-fold for 2 days and then decreased. In another human megakaryoblastic cell line, Dami, the PGD synthase increased about 10-fold by the addition of phorbol ester. Thus, the PGD synthase, which was undetectable in platelets, appeared during differentiation of megakaryoblasts tomegakaryocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 18:11:00