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Titolo:
MURINE CARDIAC PROGENITOR CELLS REQUIRE VISCERAL EMBRYONIC ENDODERM AND PRIMITIVE STREAK FOR TERMINAL DIFFERENTIATION
Autore:
ARAI A; YAMAMOTO K; TOYAMA J;
Indirizzi:
NAGOYA UNIV,DEPT CIRCULAT,ENVIRONM MED RES INST,CHIKUSA KU,FURO CHO NAGOYA AICHI 46401 JAPAN SEINAN GAKUIN UNIV,DIV BIOL FUKUOKA JAPAN
Titolo Testata:
Developmental dynamics
fascicolo: 3, volume: 210, anno: 1997,
pagine: 344 - 353
SICI:
1058-8388(1997)210:3<344:MCPCRV>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERM LAYER FORMATION; MYOSIN HEAVY-CHAIN; MOUSE EMBRYOS; ANTERIOR ENDODERM; MORPHOGENETIC MOVEMENT; PRECARDIAC MESODERM; HEART TUBES; INVITRO; SPECIFICATION; CARDIOGENESIS;
Keywords:
CARDIOMYOGENESIS; CELL INTERACTION; VISCERAL ENDODERM; PRIMITIVE STREAK; MESODERM; MOUSE EMBRYO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
A. Arai et al., "MURINE CARDIAC PROGENITOR CELLS REQUIRE VISCERAL EMBRYONIC ENDODERM AND PRIMITIVE STREAK FOR TERMINAL DIFFERENTIATION", Developmental dynamics, 210(3), 1997, pp. 344-353

Abstract

Cardiac progenitor cells in avian and amphibian embryos are known to commit to cardiac lineage during gastrulation or early neurulation. These cells require cell interaction with anterior endoderm for their differentiation into cardiomyocytes. However, little is known about cellinteraction in mammalian cardiogenesis. We investigated the staging of murine cardiomyocyte commitment and the role of cell interaction in differentiation of cardiac progenitor cells into cardiomyocytes, usingcultures of various embryonic regions at 7.25 and 7.5 days post coitum (p.c.), respectively, To evaluate the terminal differentiation of cardiac progenitor cells, we employed three parameters; expression of spontaneous beating, myosin heavy chain (MHC) protein, and cardiac-specific genes (a myosin heavy chain, Csx/Nkx2.5 and myosin light chain 2V genes), mRNAs of cardiac-specific genes were detected in 7.25-day p.c.mesoderm by RT-PCR, suggesting that the genetic specification to cardiac lineage initiated in the mesoderm by 7.25 days p.c. The 7.25-day p.c. isolated mesoderm in 48 hr culture, however, failed to differentiate into spontaneous beating cardiomyocytes and exhibited non-organizedMHC protein in 19% of these culture, In contrast, all of the 7.5-day p.c. isolated mesoderm differentiated into beating cardiomyocytes evenin 24 hr culture, The 7.25-day p.c. mesoderm associated with primitive streak increased MHC protein expression in 93% of these cultures, although they formed beating foci in 3%, The 7.25-day p.c. explants containing both visceral embryonic endoderm and primitive streak succeededin terminal differentiation into spontaneous beating cardiomyocytes. Our study suggests that cardiac progenitor cells obtain the potency tocomplete terminal differentiation autonomously at 7.5 days p.c., as aconsequence of the multistep induction by cell interactions with boththe primitive streak and visceral embryonic endoderm, following the genetic specification to cardiac lineage in the early gastrula stage. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 19:16:26