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Titolo:
AUGMENTED EXPRESSION OF A HUMAN GENE FOR 8-OXOGUANINE DNA GLYCOSYLASE(MUTM) IN B-LYMPHOCYTES OF THE DARK ZONE IN LYMPH-NODE GERMINAL-CENTERS
Autore:
KUO FC; SKLAR J;
Indirizzi:
HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT PATHOL,DIV MOL ONCOL BOSTON MA 02115 HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT PATHOL,DIV MOL ONCOL BOSTON MA 02115
Titolo Testata:
The Journal of experimental medicine
fascicolo: 9, volume: 186, anno: 1997,
pagine: 1547 - 1556
SICI:
0022-1007(1997)186:9<1547:AEOAHG>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOTYPE SWITCH RECOMBINATION; SOMATIC HYPERMUTATION; IMMUNOGLOBULIN GENES; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; ANTIBODY DIVERSITY; ESCHERICHIA-COLI; V-REGION; REPAIR PROTEIN; HUMAN-CELLS; HOT-SPOTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
F.C. Kuo e J. Sklar, "AUGMENTED EXPRESSION OF A HUMAN GENE FOR 8-OXOGUANINE DNA GLYCOSYLASE(MUTM) IN B-LYMPHOCYTES OF THE DARK ZONE IN LYMPH-NODE GERMINAL-CENTERS", The Journal of experimental medicine, 186(9), 1997, pp. 1547-1556

Abstract

B cells that mediate normal, T cell-dependent, humoral immune responses must first pass through germinal centers (GCs) within the cortex ofantigenically stimulated lymph nodes. As they move through the dark zone and then the light zone in the GC, B cells are subjected to somatic hypermutation and switch recombination within their rearranged immunoglobulin genes and also participate in a number of other processes that control development into memory cells or cells specialized for antibody secretion. To investigate the molecular mechanisms that contribute to B cell development within GCs, we constructed a recombinant DNA library enriched for cDNAs derived from human genes expressed in B cells at this site. This library was found to contain a cDNA structurally and functionally related to genes in bacteria and yeast for the DNA repair enzyme 8-oxoguanine DNA glycosylase. Northern blot analysis indicated that the human gene is expressed as two alternatively spliced messenger RNAs within GC B cells at levels greatly exceeding that found in other tissues. In situ hybridization studies revealed that expression of this gene is most abundant within the dark zones of GCs. Both thefunction and localized expression of this gene suggest that it may play a role in somatic hypermutation of immunoglobulin genes.

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Documento generato il 11/07/20 alle ore 09:51:43