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Titolo:
ENZYMES OF DELTA(1)-PYRROLINE-5-CARBOXYLATE METABOLISM IN THE CAMEL TICK HYALOMMA-DROMEDARII DURING EMBRYOGENESIS - PURIFICATION AND CHARACTERIZATION OF DELTA(1)-PYRROLINE-5-CARBOXYLATE DEHYDROGENASES
Autore:
FAHMY AS; MOHAMED SA; GIRGIS RB; ABDELGHAFFAR FA;
Indirizzi:
NATL RES CTR,DEPT MOL BIOL,TAHRIR ST CAIRO EGYPT CAIRO UNIV,FAC SCI,DEPT ZOOL CAIRO EGYPT
Titolo Testata:
Comparative biochemistry and physiology. B. Comparative biochemistry
fascicolo: 1, volume: 118, anno: 1997,
pagine: 229 - 237
SICI:
0305-0491(1997)118:1<229:EODMIT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID POOLS; ALDEHYDE DEHYDROGENASE; PROLINE; ARGININE; CONVERSION; GLUTAMATE; ORNITHINE; TISSUES; LIVER;
Keywords:
EMBRYOGENESIS; HYALOMMA DROMEDARII; METABOLISM PURIFICATION; PYRROLINE-5-CARBOXYLATE DEHYDROGENASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
A.S. Fahmy et al., "ENZYMES OF DELTA(1)-PYRROLINE-5-CARBOXYLATE METABOLISM IN THE CAMEL TICK HYALOMMA-DROMEDARII DURING EMBRYOGENESIS - PURIFICATION AND CHARACTERIZATION OF DELTA(1)-PYRROLINE-5-CARBOXYLATE DEHYDROGENASES", Comparative biochemistry and physiology. B. Comparative biochemistry, 118(1), 1997, pp. 229-237

Abstract

The activity of P5C metabolizing enzymes: OAT, P5CR, PO, and P5CD, inthe camel tick Hyalomma dromedarii has been followed throughout embryogenesis. The profiles of enzymatic activity showed clear differences in the four enzymes as the embryos grew older. During purification of P5CD to homogeneity the ion exchange chromatography stops lead to two separate forms (termed A and B) with different molecular weights (60,000-59,000 and 50,000-52,000 for the native and denatured enzymes, respectively), amino acid composition, K-m for P5C and coenzymes, varying dehydrogenase activities with different substrate specificity when supplied with various aldehyde substrates. Both P5CD A and B exhibited sharp optima at pH 7.5. The effect of different divalent cations and competitive and noncompetitive inhibitors was examined. The changes in P5C metabolizing enzymes during embryogenesis suggest that H. dromedariihas the metabolic potential to convert ornithine into proline and glutamate. (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:24:52