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Titolo:
REGIONALIZED EXPRESSION OF CD52 IN RAT EPIDIDYMIS IS RELATED TO MESSENGER-RNA POLY(A) TAIL LENGTH
Autore:
PERA I; DERR P; YEUNG CH; COOPER TG; KIRCHHOFF C;
Indirizzi:
UNIV HAMBURG,IHF INST HORMONE & FERTIL RES,GRANDWEG 64 D-22529 HAMBURG GERMANY UNIV HAMBURG,IHF INST HORMONE & FERTIL RES D-22529 HAMBURG GERMANY UNIV MUNSTER,INST REPROD MED D-4400 MUNSTER GERMANY
Titolo Testata:
Molecular reproduction and development
fascicolo: 4, volume: 48, anno: 1997,
pagine: 433 - 441
SICI:
1040-452X(1997)48:4<433:REOCIR>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; PEROXIDASE-LIKE PROTEIN; GENE-EXPRESSION; CAUDA EPIDIDYMIDIS; TESTICULAR FACTORS; MOUSE EPIDIDYMIS; MEMBRANE-GLYCOPROTEINS; SECRETORY PROTEINS; BODY-TEMPERATURE; PEPTIDE BACKBONE;
Keywords:
REGIONALIZATION; ANDROGEN EFFECTS; TEMPERATURE EFFECTS; GLYCOPEPTIDE; CARBOHYDRATE LABELING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
I. Pera et al., "REGIONALIZED EXPRESSION OF CD52 IN RAT EPIDIDYMIS IS RELATED TO MESSENGER-RNA POLY(A) TAIL LENGTH", Molecular reproduction and development, 48(4), 1997, pp. 433-441

Abstract

The regional pattern of CD52 expression in the vat epididymis was followed by Northern analyses and carbohydrate-labelling of glycoconjugates on Western blots. CD52 mRNA showed a novel aspect of regionalization, namely region-dependent length differences in its poly(A) tail. 'Short' CD52 mRNA molecules were present in all parts of this organ and also in the seminal vesicles. Additionally, the cauda epididymidis contained mRNA molecules with an extended poly(A) tail. Their appearance coincided with the occurance of the principal M-r approximate to 26 kDaglycopeptide in the cauda region, representing the CD52 product. CD52expression seemed to be regulated or modulated synergistically by androgens, temperature, and (an) unknown testicular factor(s), depending on the poly(A) tail length of its mRNA. Androgens alone exerted an effect only on molecules with 'short' poly(A) tails. They were downregulated in castrated animals, and restored to normal levels upon testosterone supplementation. However, 'long' CD52 mRNA molecules were not affected. Only if combined with the exposure of the epididymis to the elevated temperature of the abdomen, castration of animals resulted in a complete loss of the CD52 mRNA, including the 'long' cauda species. Loss of 'long' CD52 mRNA molecules was also observed when the abdominal location was combined with efferent duct ligation. This combination of treatments, however, did not affect 'short' CD52 mRNA levels. Loss of the 'long' CD52 mRNA molecules by any treatment coincided with a loss of the principal M-r approximate to 26 kDa glycopeptide from caudal protein extracts. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:06:35