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Titolo:
IMMUNOMODULATORY EFFECT OF BOVINE LACTOFERRIN PEPSIN HYDROLYSATE ON MURINE SPLENOCYTES AND PEYERS PATCH CELLS
Autore:
MIYAUCHI H; KAINO A; SHINODA I; FUKUWATARI Y; HAYASAWA H;
Indirizzi:
MORINAGA MILK IND CO LTD,NUTR SCI LAB,1-83 5-CHOME ZAMA KANAGAWA 228 JAPAN
Titolo Testata:
Journal of dairy science
fascicolo: 10, volume: 80, anno: 1997,
pagine: 2330 - 2339
SICI:
0022-0302(1997)80:10<2330:IEOBLP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR; LYMPHOCYTE-PROLIFERATION; BACTERIAL TRANSLOCATION; HUMAN-MILK; PEPTIDES; CASEIN; MUCOSAL; SYSTEM; IRON;
Keywords:
BOVINE LACTOFERRIN; PEPSIN HYDROLYSATE; IMMUNOMODULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
H. Miyauchi et al., "IMMUNOMODULATORY EFFECT OF BOVINE LACTOFERRIN PEPSIN HYDROLYSATE ON MURINE SPLENOCYTES AND PEYERS PATCH CELLS", Journal of dairy science, 80(10), 1997, pp. 2330-2339

Abstract

The effects were examined of a pepsin hydrolysate of bovine lactoferrin on the proliferation of murine splenocytes. The hydrolysate enhanced [H-3]thymidine uptake by splenocytes, but undigested bovine lactoferrin exerted an inhibitory effect. The hydrolysate had the ability to inhibit the blastogenesis that was induced by mitogens such as concanavalin A, phytohemagglutinin, and lipopolysaccharide; inhibition was similar to that with undigested lactoferrin. These results suggested thatthe hydrolysate contained both immunostimulatory and immunoinhibitorypeptides. The stimulatory effect of the hydrolysate in the absence ofmitogens was then explored in more detail using nonadherent splenocytes. The proliferative response of splenocytes to the hydrolysate was much greater in the fraction that was enriched with B cells than in thefraction that was enriched with T cells. The hydrolysate did not affect thymocyte proliferation. These data indicated that the adherent cells resembling macrophages and found among the splenocytes were not thetarget cells of the hydrolysate. The stimulatory effect of the hydrolysate was due to the activation of B cells by the hydrolysate and enhanced immunoglobulin production by splenocytes. Because the hydrolysatealso enhanced the proliferation and immunoglobulin A production of Peyer's Patch cells, the immunostimulatory effect of the hydrolysate in vivo was examined using mice that had been orally immunized with cholera toxin. The concentrations of immunoglobulin A conjugated against cholera toxin in bile and in the intestinal contents of mice fed liquid diets containing 1% (wt/vol) lactoferrin hydrolysate were greater thanthose of mice fed control diets. This result suggested that the use of the lactoferrin hydrolysate is beneficial to enhance mucosal immunity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:26:00