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Titolo:
DEVELOPMENT AND UTILIZATION OF THE RAT LYMPHOCYTE HPRT MUTATION ASSAY
Autore:
AIDOO A; MORRIS SM; CASCIANO DA;
Indirizzi:
US FDA,DEPT HLTH & HUMAN SERV,NATL CTR TOXICOL RES,DIV GENET TOXICOL JEFFERSON AR 72079
Titolo Testata:
Mutation research-reviews in mutation research
fascicolo: 2, volume: 387, anno: 1997,
pagine: 69 - 88
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETHYL-N-NITROSOUREA; DNA-SEQUENCE ANALYSIS; EXPOSED IN-VIVO; MONOFUNCTIONAL ALKYLATING-AGENTS; PERIPHERAL-BLOOD LYMPHOCYTES; RESISTANT MUTANT FREQUENCY; CIRCULATING T-LYMPHOCYTES; NORMAL HUMAN-POPULATION; SOMATIC GENE-MUTATIONS; VITAMIN-C;
Keywords:
HPRT; T-LYMPHOCYTE; RAT; VALIDATION; MUTATION SPECTRUM; DNA ADDUCT;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
154
Recensione:
Indirizzi per estratti:
Citazione:
A. Aidoo et al., "DEVELOPMENT AND UTILIZATION OF THE RAT LYMPHOCYTE HPRT MUTATION ASSAY", Mutation research-reviews in mutation research, 387(2), 1997, pp. 69-88

Abstract

Much of the recent progress in the field of genetic toxicology has come from an increased understanding of the molecular and cellular biology of the mammalian organism. Most prominent has been the ability to detect and quantify somatic mutation and relate the nature of the mutation to the specific type of chemical damage. Building upon the foundation of the human lymphocyte hypoxanthine guanine phosphoribosyl transferase (hprt) system, and later, the mouse hprt system, methods for thedetection and quantification of hprt mutations in rat lymphocytes were developed. These methods are described in this report as is the ongoing validation of the assay. Additionally, the characterization of therecovered mutants and a comparison of the mutation spectrum in the rat lymphocyte system to the spectrum in cancer genes, such as H-ras andp53, and the spectrum in transgenic systems, such as IacI, are included. The development of the rat lymphocyte hprt system and validation of the assay at the molecular level, provide an effective and reliable measure of genetic damage in an in vivo system which is readily comparable to measurement of genetic damage in the human. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 08:48:58