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Titolo:
NOVEL ROLE FOR THE PERITROPHIC MATRIX IN PROTECTING LEISHMANIA FROM THE HYDROLYTIC ACTIVITIES OF THE SAND FLY MIDGUT
Autore:
PIMENTA PFP; MODI GB; PEREIRA ST; SHAHABUDDIN M; SACKS DL;
Indirizzi:
NIAID,PARASIT DIS LAB,NIH,BLDG 4,RM 126 BETHESDA MD 20892 NIAID,PARASIT DIS LAB,NIH BETHESDA MD 20892 UNIV ESTADUAL NORTE FLUMINENSE,CTR BIOCENCIAS & BIOTECHNOL,LAB BIOL CELULAR & TECIDUAL BR-28015620 RIO JANEIRO BRAZIL
Titolo Testata:
Parasitology
, volume: 115, anno: 1997,
parte:, 4
pagine: 359 - 369
SICI:
0031-1820(1997)115:<359:NRFTPM>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHLEBOTOMUS-PAPATASI DIPTERA; AEDES-AEGYPTI L; MEXICANA-AMAZONENSIS; VECTOR COMPETENCE; LIPOPHOSPHOGLYCAN; PSYCHODIDAE; MOSQUITO; MEMBRANE; TRANSMISSION; INSECTS;
Keywords:
CHITINASE; LEISHMANIA; PERITROPHIC MEMBRANE; SAND FLY; ALLOSAMIDIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
P.F.P. Pimenta et al., "NOVEL ROLE FOR THE PERITROPHIC MATRIX IN PROTECTING LEISHMANIA FROM THE HYDROLYTIC ACTIVITIES OF THE SAND FLY MIDGUT", Parasitology, 115, 1997, pp. 359-369

Abstract

The role of the peritrophic matrix (PM) in the development of Leishmania major infections in a natural vector, Phlebotomus papatasi, was investigated by addition of exogenous chitinase to the bloodmeal, which completely blocked PM formation. Surprisingly, the absence of the PM was associated with the loss of midgut infections. The chitinase was not directly toxic to the parasite, nor were midgut infections lost due to premature expulsion of the bloodmeal. Most parasites were killed inchitinase-treated flies within the first 4 h after feeding. Substantial early killing was also observed in control flies, suggesting that the lack of PM exacerbates lethal conditions which normally exist in the blood-fed midgut. Early parasite mortality was reversed by soybean trypsin inhibitor. Allosamadin, a specific inhibitor of chitinase, led to a thickening of the PM, and also prevented the early parasite mortality seen in infected flies. Susceptibility to gut proteases was extremely high in transitional-stage parasites, while amastigotes and fullytransformed promastigotes were relatively resistant. A novel role forthe PM in promoting parasite survival is suggested, in which the PM creates a barrier to the rapid diffusion of digestive enzymes, and limits the exposure of parasites to these enzymes during the time when they are especially vulnerable to proteolytic damage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:54:48