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Titolo:
GENOMIC DNA REPEAT FROM LEISHMANIA (VIANNIA) BRAZILIENSIS (VENEZUELANSTRAIN) CONTAINING SIMPLE REPEATS AND MICROSATELLITES
Autore:
RODRIGUEZ N; DELIMA H; RODRIGUEZ A; BREWSTER S; BARKER DC;
Indirizzi:
UNIV CAMBRIDGE,DEPT PATHOL,MOLTENO LABS,MRC OUTSTN NIMR,TENNIS COURT RD CAMBRIDGE CB2 1QP ENGLAND UNIV CAMBRIDGE,DEPT PATHOL,MOLTENO LABS,MRC OUTSTN NIMR CAMBRIDGE CB21QP ENGLAND CENT UNIV VENEZUELA,INST BIOMED CARACAS VENEZUELA
Titolo Testata:
Parasitology
, volume: 115, anno: 1997,
parte:, 4
pagine: 349 - 358
SICI:
0031-1820(1997)115:<349:GDRFL(>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENES; SEQUENCE; DONOVANI; COMPLEX; POLYMORPHISM; ARRANGEMENT; PERUVIANA; FRAGMENTS;
Keywords:
LEISHMANIA (V) BRAZILIENSIS; MICROSATELLITE; DNA SEQUENCE; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
N. Rodriguez et al., "GENOMIC DNA REPEAT FROM LEISHMANIA (VIANNIA) BRAZILIENSIS (VENEZUELANSTRAIN) CONTAINING SIMPLE REPEATS AND MICROSATELLITES", Parasitology, 115, 1997, pp. 349-358

Abstract

In this paper the Leishmania (Viannia) braziliensis complex is defined as containing all species of the actual subgenus Viannia. Organisms of the L. (V) braziliensis complex are the causative agents of localized human cutaneous and mucocutaneous leishmaniasis in South America, much of Central America and some ares of North America. In our search for better species and subspecies diagnostic probes we focused our research on repetitive DNA, since it provides a greater number of target sites for hybridization. In this work we report the isolation and sequencing of a 1.8 kb DNA region, LbJ38, which is probably tandemly repeated or dispersed at least 4 times along one chromosome and is naturallypresent in L. (V) braziliensis genomic DNA. This region contains microsatellites and simple repeat DNA sequences and was isolated by screening a genomic DNA cosmid library with complex-and species-specific probes. No homology was found with other Leishmania microsatellite or repetitive DNA. The utility of this repetitive sequence and primers derived from it in the identification of L. (V) braziliensis is demonstrated. As far as we are aware, this is the first report of sequence characterized repetitive microsatellite and GC rich simple repeat DNA from the nuclear genome of New World Leishmania.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 14:22:06