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Titolo:
EFFECTS OF PARATHION ON MEMBRANE ORGANIZATION AND ITS IMPLICATIONS FOR THE MECHANISMS OF TOXICITY
Autore:
ANTUNESMADEIRA MC; VIDEIRA RA; MADEIRA VMC;
Indirizzi:
DEPT ZOOL,CTR BIOL CELULAR P-3049 COIMBRA PORTUGAL DEPT ZOOL,CTR BIOL CELULAR P-3049 COIMBRA PORTUGAL
Titolo Testata:
Biochimica et biophysica acta. Biomembranes
fascicolo: 1, volume: 1190, anno: 1994,
pagine: 149 - 154
SICI:
0005-2736(1994)1190:1<149:EOPOMO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUIDITY; CHOLESTEROL; DDT; DIPHENYLHEXATRIENE; INSECTICIDES; POLARIZATION; BILAYERS; LINDANE; PROBE;
Keywords:
PARATHION; CHOLESTEROL; FLUORESCENT PROBE; MEMBRANE ORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Antunesmadeira et al., "EFFECTS OF PARATHION ON MEMBRANE ORGANIZATION AND ITS IMPLICATIONS FOR THE MECHANISMS OF TOXICITY", Biochimica et biophysica acta. Biomembranes, 1190(1), 1994, pp. 149-154

Abstract

The effects of the organophosphorus insecticide parathion (O,O-diethyl O-(p-nitrophenyl)phosphorothioate) on the physical state of synthetic and native membranes was investigated by fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH), probing the bilayer core, and by its anionic propionic acid derivative (DPH-PA), probing the outer regions of the bilayer. Parathion disorders the gel phase of liposomes reconstituted with dimyristoylphosphatidylcholine (DMPC), broadening the transition profile and shifting the temperature midpoint of the phase transition, as detected by both probes. The insecticide strongly orders the fluid phase either in the hydrophobic core or in the outer regions of the membrane, as evaluated by DPH and DPH-PA, respectively. These ordering effects of parathion were further confirmed in fluid models of egg-yolk phosphatidylcholine. Parathion increases to some extentthe ordering promoted by cholesterol in DMPC bilayers, but high cholesterol concentrations (greater than or equal to 130 mol%) prevent parathion interaction. The results in native membranes correlate reasonably with those obtained in models of synthetic lipids. Thus, parathion does not exert detectable effects in cholesterol-rich membranes, namely, erythrocytes, but moderate ordering effects of parathion are detected by both probes in brain microsomes, i.e., membranes with a lower content of cholesterol. Again, in agreement with the models of synthetic lipids, pronounced ordering effects of parathion are detected in cholesterol-poor membranes, e.g., sarcoplasmic reticulum and mitochondria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:53:38