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Titolo:
VECTORS BASED ON SEMLIKI-FOREST-VIRUS FOR RAPID AND EFFICIENT GENE-TRANSFER INTO NON-ENDOTHELIAL CARDIOVASCULAR CELLS - COMPARISON TO ADENOVIRUS
Autore:
ROKS AJM; PINTO YM; PAUL M; PRIES F; STULA M; ESCHENHAGEN T; ORZECHOWSKI HD; GSCHWENDT S; WILSCHUT J; VANGILST WH;
Indirizzi:
FREE UNIV BERLIN,DEPT CLIN PHARMACOL,HINDENBURGDAMM 30 D-12200 BERLINGERMANY FREE UNIV BERLIN,DEPT CLIN PHARMACOL D-12200 BERLIN GERMANY UNIV GRONINGEN,DEPT CLIN PHARMACOL GRONINGEN NETHERLANDS UNIV GRONINGEN,DEPT PHYSIOL CHEM GRONINGEN NETHERLANDS UNIV HAMBURG,DEPT PHARMACOL HAMBURG GERMANY
Titolo Testata:
Cardiovascular Research
fascicolo: 3, volume: 35, anno: 1997,
pagine: 498 - 504
SICI:
0008-6363(1997)35:3<498:VBOSFR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; IN-VIVO; EXPRESSION VECTORS; REPLICATION; GENERATION; PARTICLES; VITRO;
Keywords:
GENE TRANSFER; CELL SPECIFIC; VASCULAR SMOOTH MUSCLE CELL; ENDOTHELIAL CELL; CARDIAC MYOCYTE; RECOMBINANT SEMLIKI FOREST VIRUS; RECOMBINANT ADENOVIRUS; CELL CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
A.J.M. Roks et al., "VECTORS BASED ON SEMLIKI-FOREST-VIRUS FOR RAPID AND EFFICIENT GENE-TRANSFER INTO NON-ENDOTHELIAL CARDIOVASCULAR CELLS - COMPARISON TO ADENOVIRUS", Cardiovascular Research, 35(3), 1997, pp. 498-504

Abstract

Objective: Replication-deficient, recombinant adenovirus is used as acarrier for gene transfer, but it is unspecific and the onset of transgene expression is relatively late. Here, we evaluated the efficiencyand selectivity of gene transfer mediated by recombinant Semliki Forest virus (SFV). Methods: We compared the efficiency of a SFV-based vector with an adenoviral vector, using LacZ as a reporter gene. Firstly,the affinity for vascular smooth muscle cells, endothelial cells and cardiac myocytes was assessed. Secondly, we compared the time course of LacZ expression and cytotoxicity in vascular smooth muscle cells. Results: The SFV-based vector infects vascular smooth muscle cells and cardiomyocytes as efficiently as adenovirus. In contrast to adenovirus,SFV hardly transfers LacZ to endothelial cells (2.6% or less). SFV-mediated expression was visible after 1 h, reaching a maximum after 6 h. In contrast, adenovirus-mediated expression became visible after 6 h,and reached a maximum after 48-72 h. Both vectors were cytotoxic. Conclusions: We demonstrate that SFV efficiently transfers LacZ to vascular smooth muscle cells and cardiomyocytes, but not to endothelial. cells. In contrast, adenovirus causes efficient transgene expression in all cell types tested. Furthermore, SFV-mediated expression is faster than adenovirus-mediated expression. Therefore, SFV-mediated gene transfer may be a suitable alternative to adenovirus, providing a fast expression in non-endothelial cardiovascular cell types. (C) 1997 ElsevierScience B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:18:47