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Titolo:
TISSUE DISTRIBUTION OF THE P2X(7) RECEPTOR
Autore:
COLLO G; NEIDHART S; KAWASHIMA E; KOSCOVILBOIS M; NORTH RA; BUELL G;
Indirizzi:
GLAXO WELLCOME RES & DEV LTD,GENEVA BIOMED RES INST,PLAN LES OUATES CH-1228 GENEVA SWITZERLAND GLAXO WELLCOME RES & DEV LTD,GENEVA BIOMED RES INST CH-1228 GENEVA SWITZERLAND
Titolo Testata:
Neuropharmacology
fascicolo: 9, volume: 36, anno: 1997,
pagine: 1277 - 1283
SICI:
0028-3908(1997)36:9<1277:TDOTPR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GATED ION CHANNELS; EXTRACELLULAR ATP; P-2X RECEPTOR; MICROGLIAL CELLS; PLASMA-MEMBRANE; MOUSE-BRAIN; PURINOCEPTORS; RAT; RELEASE;
Keywords:
IN SITU HYBRIDIZATION; IMMUNOHISTOCHEMISTRY; P2X(7) RECEPTOR; MACROPHAGE; MONOCYTE; MICROGLIA; PURINERGIC RECEPTORS; ATP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
G. Collo et al., "TISSUE DISTRIBUTION OF THE P2X(7) RECEPTOR", Neuropharmacology, 36(9), 1997, pp. 1277-1283

Abstract

The P2X(7) receptor is a bifunctional molecule. The binding of ATP induces within milliseconds the opening of a channel selective for smallcations, and within seconds a larger pore opens which allows permeation by molecules as large as propidium dyes (629 Da). In situ hybridization using a digoxigenin-labelled riboprobe, and immunohistochemistry using an antibody raised against a C-terminal peptide sequence, were used to determine the distribution of the P2X(7) receptor mRNA and protein in rat and mouse tissues and cell lines. The brain of newborn ratsshowed a 6 kb RNA by Northern blotting, but this was not detectable in adult brain. By in situ hybridization and immunohistochemistry, there was heavy labelling of ependymal cells in both newborn and adult brain, but the brain parenchyma showed no labelling. However, P2X(7) receptor-immunoreactive cells appeared in the penumbral region around an area of necrosis evoked by prior occlusion of the middle cerebral artery, suggesting expression of the receptor by activated microglia. NTW8 cells, a mouse microglial cell Line, strongly expressed the P2X(7) receptor mRNA and protein. The P2X(7) receptor mRNA and protein were alsoobserved in the majority of bone marrow cells, including those separately identified by their expression of other antigens as granulocytes,monocyte/macrophages and B lymphocytes. The expression of P2X(7) receptor by brain macrophages rather than neurons would be consistent witha role in brain repair following inflammation, infarction or immune insult. (C) 1997 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:09:13