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Titolo:
THYMIDYLATE SYNTHASE ACTIVITY, FOLATES, AND GLUTATHIONE SYSTEM IN HEAD AND NECK-CARCINOMA AND ADJACENT TISSUES
Autore:
PARISE O; JANOT F; LUBOINSKI B; MASSAAD L; ALBIN N; TOUSSAINT C; VERJUS MA; BONNAY M; GOUYETTE A; CHABOT GG;
Indirizzi:
INST GUSTAVE ROUSSY,PHARMACOTOXICOL & PHARMACOGENET LAB,PAVILLON RECH2 F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,PHARMACOTOXICOL & PHARMACOGENET LAB,PAVILLON RECH2 F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,DEPT HEAD & NECK SURG F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,CLIN BIOL LAB F-94805 VILLEJUIF FRANCE
Titolo Testata:
Head & neck
fascicolo: 2, volume: 16, anno: 1994,
pagine: 158 - 164
SICI:
1043-3074(1994)16:2<158:TSAFAG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
S-TRANSFERASES; BIOCHEMICAL MODULATION; COLORECTAL-CARCINOMA; RESISTANCE; 5-FLUOROURACIL; CHEMOTHERAPY; CANCER; EXPRESSION; TUMOR; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
O. Parise et al., "THYMIDYLATE SYNTHASE ACTIVITY, FOLATES, AND GLUTATHIONE SYSTEM IN HEAD AND NECK-CARCINOMA AND ADJACENT TISSUES", Head & neck, 16(2), 1994, pp. 158-164

Abstract

Background. Head and neck squamous cell carcinomas (HNSCC) present variable aggressiveness and chemosensitivity. Because the glutathione (GSH) system and thymidylate synthase (TS) are involved in the resistance to the main drugs used in HNSCC (cisplatin and 5-FU), we studied these systems in tumors and normal mucosae. Methods. Tumor samples and normal adjacent mucosae were collected from 37 untreated HNSCC patients. GSH and glutathione S-transferase (GST) activity were assayed by spectrophotometry, whereas TS activity and folates were determined by radioassays. Results. Mean GSH levels were higher in tumors (15.2 +/-8.2 nmol/mg protein) than in mucosae (8.3 +/- 4.1 nmol/mg protein) (p = 0.005, paired t test). GST activity was also higher in tumors (394 +/- 194 nmol/min/mg protein) than in mucosae (261 +/- 132 nmol/min/mg protein) (p = 0.0003). TS activity was markedly higher in tumors (9.2 +/- 21.5 pmol/min/mg protein) (p = 0.0001). Folate levels in tumors and mucosae were similar (1.2 +/- 1.1 and 0.8 +/- 0.9 pmol/mg protein, respectively; p = 0.1, NS). In relation to clinical stage and tumor size, a statistical difference was found in GSH and GST values between tumors and mucosae for stage IV and T3/T4. The increase in tumor TS compared to that of mucosae was significant for all clinical stages, tumor sizesand nodal involvement. Conclusions. These data enhance our understanding of the enzymatic systems involved in cisplatin and 5-fluorouracil (5-FU) resistance in HNSCC and normal mucosae and may help to elucidate tumor behavior and interpatient differences in drug sensitivity.

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Documento generato il 02/12/20 alle ore 14:02:38