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Titolo:
SUBSTRATE AND TEMPERATURE-DEPENDENCE OF DNA PHOTOLYASE REPAIR ACTIVITY EXAMINED WITH ULTRAFAST SPECTROSCOPY
Autore:
LANGENBACHER T; ZHAO XD; BIESER G; HEELIS PF; SANCAR A; MICHELBEYERLE ME;
Indirizzi:
TECH UNIV MUNICH,INST PHYS CHEM,LICHTENBERGSTR 4 D-85748 GARCHING GERMANY TECH UNIV MUNICH,INST PHYS CHEM D-85748 GARCHING GERMANY N E WALES INST WREXHAM LL11 2AW WALES UNIV N CAROLINA,DEPT BIOCHEM & BIOPHYS CHAPEL HILL NC 27599
Titolo Testata:
Journal of the American Chemical Society
fascicolo: 43, volume: 119, anno: 1997,
pagine: 10532 - 10536
SICI:
0002-7863(1997)119:43<10532:SATODP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; SKIN-CANCER; ELECTRON-TRANSFER; DIMER; PERSPECTIVE; SUNLIGHT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
T. Langenbacher et al., "SUBSTRATE AND TEMPERATURE-DEPENDENCE OF DNA PHOTOLYASE REPAIR ACTIVITY EXAMINED WITH ULTRAFAST SPECTROSCOPY", Journal of the American Chemical Society, 119(43), 1997, pp. 10532-10536

Abstract

The kinetics and energetics of the electron transfer reactions occurring in the enzyme substrate complex of DNA photolyase have been studied by transient absorption spectroscopy with picosecond time resolutionin the temperature range 275-90 K. The results can be summarized as follows: (i) The lifetime of (1)(FADH(-)) depends not only on the presence of the substrate but also on its nature, e.g. the electron transfer from (1)(FADH(-)) to thymine dimers T >$($) over bar T and to thymine-uracil dimers T >$($) over bar U is slower by a factor of 2 to 3 compared to that of U >$($) over bar U and U >$($) over bar T. This feature is attributed to the enlarged electronic coupling between (1)(FADH(-)) and the respective dimers in the absence of the methyl group inthe C5 position of the 5'-uracil. (ii) The temperature dependence of the quantum yield was studied, and from this the activation energy forthe overall repair process has been estimated to be E-A=0.45 +/- 0.1 eV.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 20:55:39