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Titolo:
POLYMERASE CHAIN-REACTION ON CEREBROSPINAL-FLUID CELLS IN THE DETECTION OF LEPTOMENINGEAL INVOLVEMENT BY B-CELL LYMPHOMA AND LEUKEMIA - A NOVEL STRATEGY AND ITS IMPLICATIONS
Autore:
GALOIN S; DASTE G; APOIL PA; CHOLLET F; RODA D; BLANCHER A; DELSOL G; CHITTAL S; ALSAATI T;
Indirizzi:
CHU PURPAN,CTR IMMUNOPATHOL & GENET HUMAINE,CNRS,CIGH,AVE GRANDE BRETAGNE F-31300 TOULOUSE FRANCE CHU PURPAN,CTR IMMUNOPATHOL & GENET HUMAINE,CNRS,CIGH F-31300 TOULOUSE FRANCE CHU PURPAN,DEPT PATHOL F-31300 TOULOUSE FRANCE CHU PURPAN,DEPT IMMUNOL F-31300 TOULOUSE FRANCE CHU PURPAN,DEPT NEUROL F-31300 TOULOUSE FRANCE MEM UNIV NEWFOUNDLAND ST JOHNS NF CANADA
Titolo Testata:
British Journal of Haematology
fascicolo: 1, volume: 99, anno: 1997,
pagine: 122 - 130
SICI:
0007-1048(1997)99:1<122:PCOCCI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; ACUTE LYMPHOBLASTIC-LEUKEMIA; NON-HODGKINS LYMPHOMA; MINIMAL RESIDUAL DISEASE; FOLLICULAR LYMPHOMAS; GENE REARRANGEMENT; IMMUNOGLOBULIN; CLONALITY; DIAGNOSIS; PCR;
Keywords:
LYMPHOMA/LEUKAEMIA; CEREBROSPINAL FLUID (CSF); IGH GENE REARRANGEMENT; PCR; CYTOLOGIC ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S. Galoin et al., "POLYMERASE CHAIN-REACTION ON CEREBROSPINAL-FLUID CELLS IN THE DETECTION OF LEPTOMENINGEAL INVOLVEMENT BY B-CELL LYMPHOMA AND LEUKEMIA - A NOVEL STRATEGY AND ITS IMPLICATIONS", British Journal of Haematology, 99(1), 1997, pp. 122-130

Abstract

In the search of B-cell lymphoma/leukaemia dissemination to cerebrospinal fluid (CSF), we used the highly sensitive semi-nested PCR (snPCR)for the analysis of IgH gene rearrangements. This method detects a rearranged IgH gene from a single B lymphocyte which may or may not represent the neoplastic B-cell population. We therefore performed multiple snPCR (three to five) experiments on the same CSP sample, postulating that the detection of a band of the same size and sequence in different PCR runs was highly indicative of a clonal population. 17 consecutive cases with a differential diagnosis of primary (PCNSL) (n=10) or secondary (SCNSL) (n=7) CNS lymphoma or leukaemia were investigated by the new strategy. The clonal nature of the B-cell population was confirmed in 3/10 of suspected PCNSL, and in six other cases the PCR study was indicative of reactive lymphocytosis. One case revealed a clonal B-cell population in the clinical context of an autoimmune disorder. Evidence of clonal B-cell population was found in 4/7 of suspected SCNSL. In one of these cases the detected band and its sequence proved identical to that of the primary nodal lymphoma. We believe that the evaluation of B-cell clonality in CSF requires multiple snPCR amplificationon the same sample to compare the size of the products and, if necessary, the DNA sequences to ascertain the diagnosis of malignancy in equivocal cytologic and clinical findings.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:53:23