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Titolo:
EXPRESSION AND FUNCTION OF OUTWARD K+ CHANNELS INDUCED BY LIPOPOLYSACCHARIDE IN MICROGLIA
Autore:
PYO H; CHUNG S; JOU I; GWAG B; JOE EH;
Indirizzi:
AJOU UNIV,SCH MED,DEPT PHARMACOL SUWON 442749 SOUTH KOREA AJOU UNIV,SCH MED,DEPT PHARMACOL SUWON 442749 SOUTH KOREA CHUNG ANG UNIV,COLL MED,DEPT PHYSIOL SEOUL 156756 SOUTH KOREA
Titolo Testata:
Molecules and cells
fascicolo: 5, volume: 7, anno: 1997,
pagine: 610 - 614
SICI:
1016-8478(1997)7:5<610:EAFOOK>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTASSIUM CHANNELS; PERITONEAL-MACROPHAGES; AMEBOID MICROGLIA; RAT MICROGLIA; B-LYMPHOCYTES; CELLS; PROTEIN; BRAIN; IDENTIFICATION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
H. Pyo et al., "EXPRESSION AND FUNCTION OF OUTWARD K+ CHANNELS INDUCED BY LIPOPOLYSACCHARIDE IN MICROGLIA", Molecules and cells, 7(5), 1997, pp. 610-614

Abstract

Cultured microglia exposed to lipopolysaccharide (LPS) express outward K+ currents. These currents are due to the expression of new K+ channels detected by antibodies against Kv1.5, a shaker type delayed rectifier. We examined whether the K+ currents are involved in microglial activation. The amount of nitrite, converted from nitric oxide (NO), was measured as an indication of microglial activation [Chao, C. C., Hu,S., Molitor, T. W., Shaskan, 1E. G., and Peterson, P. K. (1992) J. Immunol. 149, 2730-2741]. Nitrite was detected starting from 24 h after LPS treatment and continuously increased over 4 days. Contrary to this, in the presence of 4-aminopyridine (4-AP, 2 mM), a blocker of outward K+ currents, nitrite production was reduced to less than 50%, However, the treatment of 4-AP 24 h after LPS did not reduce nitrite production. Other K+ channel blockers having a less blocking effect on outward K+ currents, such as 5 mM tetraethylammonium (TEA) and 1 mM Cs+, hadlittle effect. The present study shows that the outward K+ channels induced by LPS are immunologically related to Kv1.5 and also suggests that these channels are required for microglial activation, particularly for the initiation of the activation process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:36:37