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Titolo:
COMPARISON OF SERUM-PROTEIN ELECTROPHORESIS BY AGAROSE-GEL AND CAPILLARY-ZONE-ELECTROPHORESIS IN A CLINICAL SETTING
Autore:
JOLLIFF CR; BLESSUM CR;
Indirizzi:
NEBRASKA WESLEYAN UNIV,IMMUNOL PHYS LAB SERV LINCOLN NE 68500 BECKMAN INSTRUMENTS INC BREA CA 00000
Titolo Testata:
Electrophoresis
fascicolo: 10, volume: 18, anno: 1997,
pagine: 1781 - 1784
SICI:
0173-0835(1997)18:10<1781:COSEBA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Keywords:
CAPILLARY ZONE ELECTROPHORESIS; SERUM PROTEIN ELECTROPHORESIS; IMMUNOFIXATION ELECTROPHORESIS; IMMUNOSUBTRACTION; DYSPROTEINEMIA; PARAPROTEINEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C.R. Jolliff e C.R. Blessum, "COMPARISON OF SERUM-PROTEIN ELECTROPHORESIS BY AGAROSE-GEL AND CAPILLARY-ZONE-ELECTROPHORESIS IN A CLINICAL SETTING", Electrophoresis, 18(10), 1997, pp. 1781-1784

Abstract

Capillary zone electrophoresis (CZE) offers the potential for automating serum protein electrophoretic analysis traditionally performed on standard thin-layer agarose gels. The following describes the use of CZE compared to agarose gel electrophoresis (AGE) for the detection of dysproteinemia and paraproteinemia in a clinical study involving 240 patients. The study includes within-run and between-run reproducibilitydata on the Paragon CZE((R)) 2000 Clinical Capillary Electrophoresis System, in addition to concordance data between the two methodologies. Paraprotein quantitation studies comparing AGE versus CZE were also performed. Reproducibility for the automated CZE system was superior tothe AGE system. Improved reproducibility for the CZE method is largely due to measuring protein absorbance directly at 214 nm versus the traditional AGE method that measures the amount of dye adsorbed to protein. Reproducibility data as percent coefficient of variance (% CV) forthe five classic bands in a normal control serum for between-run precision ranged from 1.2 to 4.5% for CZE compared to AGE, which ranged from 3.8 td 8.0% CV. Concordance studies between AGE and CZE involving dysproteinemias including hypogammaglobulinemia, polyclonal and monoclonal gammopathies, acute and chronic inflammation, nephrosis, hepatodegenerative disease, cirrhosis, and iron deficiency anemia showed 96% agreement. Paraprotein classification, which compared the CZE immunosubtraction method to immunofixation electrophoresis (IFE) on agarose, showed 100% agreement. Certain dysproteinemias involving beta lipoproteinwere in partial concordance due to the inability of the CZE procedureto detect this component. Detection limits for monoclonal gammopathies, providing they were not comigrating with other proteins, were IgG 50 mg/dL, IgM 75 mg/dL, and IgA 75 mg/dL. Paraprotein quantitative studies between the two methods showed less than a +/- 0.2 g/dL variation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:43:50