Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
NUCLEOTIDE-SEQUENCE OF THE PORPHYROMONAS-GINGIVALIS W83 RECA HOMOLOG AND CONSTRUCTION OF A RECA-DEFICIENT MUTANT
Autore:
FLETCHER HM; MORGAN RM; MACRINA FL;
Indirizzi:
LOMA LINDA UNIV,DEPT MOL GENET & MICROBIOL LOMA LINDA CA 92350 VIRGINIA COMMONWEALTH UNIV,DEPT MICROBIOL & IMMUNOL RICHMOND VA 23298
Titolo Testata:
Infection and immunity
fascicolo: 11, volume: 65, anno: 1997,
pagine: 4592 - 4597
SICI:
0019-9567(1997)65:11<4592:NOTPWR>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; ESCHERICHIA-COLI; SUPEROXIDE-DISMUTASE; POLYMORPHONUCLEAR LEUKOCYTES; MICROBIAL PATHOGENESIS; PERIODONTAL-DISEASES; HOST-DEFENSE; GENE; EXPRESSION; FRAGILIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
H.M. Fletcher et al., "NUCLEOTIDE-SEQUENCE OF THE PORPHYROMONAS-GINGIVALIS W83 RECA HOMOLOG AND CONSTRUCTION OF A RECA-DEFICIENT MUTANT", Infection and immunity, 65(11), 1997, pp. 4592-4597

Abstract

Degenerate oligonucleotide primers were used in PCR to amplify a region of the recA homolog-from Porphyromonas gingivalis W83., The resulting PCR fragment was used as a probe to identify a recombinant lambda DASH phage (L10) carrying the P. gingivalis recA homolog. The recA homolog was localized to a 2.1-kb BamHI fragment. The nucleotide sequence of this 2.1-kb fragment was determined, and a 1.02-kb open reading frame (341 amino acids) was detected. The predicted amino acid sequence was strikingly similar (90% identical residues) to the RecA protein from Bacteroides fragilis, No SOS box, characteristic of LexA-regulated promoters, was found in the 5' upstream region of the P. gingivalis recA homolog, In both methyl methanesulfonate and UV survival experimentsthe recA homolog from P. gingivalis complemented the recA mutation ofEscherichia coli HB101. The cloned P. gingivalis recA gene was insertionally inactivated with the ermF-ermAM antibiotic resistance cassetteto create a recA-deficient mutant (FLL33) by allelic exchange. The recA-deficient mutant was significantly more sensitive to UV irradiationthan the wild-type strain, W83. W83 and FLL33 showed the same level of virulence in in vivo experiments using a mouse model. These results suggest that the recA gene in P. gingivalis W83 plays the expected role of repairing DNA damage caused by UV irradiation. However, inactivation of this gene did not alter the virulence of P. gingivalis in the mouse model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:11:31