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Titolo:
MODIFICATIONS OF CARBOHYDRATE RESIDUES AND ZP2 AND ZP3 GLYCOPROTEINS IN THE MOUSE ZONA-PELLUCIDA AFTER FERTILIZATION
Autore:
AVILES M; JABER L; CASTELLS MT; BALLESTA J; KAN FWK;
Indirizzi:
QUEENS UNIV,FAC MED,DEPT ANAT & CELL BIOL KINGSTON ON CANADA QUEENS UNIV,FAC MED,DEPT ANAT & CELL BIOL KINGSTON ON CANADA UNIV MURCIA,SCH MED,DEPT CELL BIOL MURCIA SPAIN
Titolo Testata:
Biology of reproduction
fascicolo: 5, volume: 57, anno: 1997,
pagine: 1155 - 1163
SICI:
0006-3363(1997)57:5<1155:MOCRAZ>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM RECEPTOR ACTIVITY; RICINUS-COMMUNIS AGGLUTININ; O-LINKED OLIGOSACCHARIDES; DATURA-STRAMONIUM LECTIN; ACETYL-D-GALACTOSAMINE; CYTOCHEMICAL CHARACTERIZATION; ULTRASTRUCTURAL-LOCALIZATION; MONOCLONAL-ANTIBODIES; LOTUS-TETRAGONOLOBUS; BINDING-PROPERTIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
M. Aviles et al., "MODIFICATIONS OF CARBOHYDRATE RESIDUES AND ZP2 AND ZP3 GLYCOPROTEINS IN THE MOUSE ZONA-PELLUCIDA AFTER FERTILIZATION", Biology of reproduction, 57(5), 1997, pp. 1155-1163

Abstract

Oligosaccharide side chains of zona pellucida (ZP) glycoproteins playa key role in the sperm-egg interaction phenomena during fertilization. In the present study, modifications of the ZP glycoproteins during the fertilization process in the mouse were studied by the lectin-goldtechnique and immunocytochemistry in conjunction with quantitative analysis. Binding of PNA, RCA I, DSA, LFA, MAA, AAA, and anti-ZP2 and anti-ZP3 antibodies was observed throughout the ZP of both unfertilized and fertilized eggs. However, HPA and BSAIB(4) labeling was found onlyin the inner region of the ZP. After neuraminidase treatment (Neu), HPA showed an affinity for the entire ZP. Labeling by LFA, WGA, MAA, PNA, BSAIB(4), and AAA decreased in the ZP of fertilized eggs; however, there was an increase in the binding of RCA I. HPA and Neu-HPA increased only in the inner region of the ZP. Immunoreactivity to antibodies against ZP2 and ZP3 also decreased after fertilization. The present results demonstrate that 1) terminal carbohydrate residues contained in the ZP glycoproteins are modified after fertilization and 2) inner andouter regions of the ZP contain different oligosaccharide side chains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 01:19:41