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Titolo:
AN EXAMINATION OF THE PEROXIDASES FROM LUPINUS-ALBUS L HYPOCOTYLS
Autore:
JACKSON P; RICARDO CPP;
Indirizzi:
UNIV NOVA LISBOA,INST TECNOL QUIM & BIOL,APARTADO 127 P-2780 OEIRAS PORTUGAL INST SUPER AGRON,DEPT BOT & ENGN BIOL P-1399 LISBON PORTUGAL
Titolo Testata:
Planta
fascicolo: 3, volume: 194, anno: 1994,
pagine: 311 - 317
SICI:
0032-0935(1994)194:3<311:AEOTPF>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY-CELL WALL; ACIDIC ISOPEROXIDASES; SOMATIC EMBRYOGENESIS; BASIC ISOPEROXIDASES; PHENOLIC COMPONENTS; CROSS-LINKING; ELECTROPHORESIS; LIGNIFICATION; LOCALIZATION; RESOLUTION;
Keywords:
CELL WALL; CONFORMER (PEROXIDASE); INDOLE-3-ACETIC ACID (OXIDASES); LEGUMINOSAE; LUPINUS (HYPOCOTYL); PEROXIDASE (ACIDIC, BASIC);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
P. Jackson e C.P.P. Ricardo, "AN EXAMINATION OF THE PEROXIDASES FROM LUPINUS-ALBUS L HYPOCOTYLS", Planta, 194(3), 1994, pp. 311-317

Abstract

Peroxidases (EC 1.11.1.7) from hypocotyls of Lupinus albus L. cv. RioMaior have been characterised using one- and two-dimensional, native electrophoretic techniques. Data are presented showing the complexity in charge and molecular size or shape of these peroxidases. We report the finding of a new acidic peroxidase and several new basic peroxidases in these hypocotyls, and of their stability to treatments considered to break ligand-induced variants and conformational variants derivedfrom differences in polypeptide folding. Densitometric data demonstrate that these new peroxidases contribute up to 60% of the total peroxidase activity in hypocotyls. Studies of intercellular fluid, cell-walland soluble fractions, with assays of purity were conducted in an attempt to define the subcellular locations of these additional peroxidases. The acidic form (pI 4.1) is greatly enriched in soluble fractions,three of the basic peroxidases (pIs 9.5, 9.7 and >9.7) are strongly associated to the cell wall, ad a minor, basic component (pI 9.7) is enriched in the intercellular fluid. Individual peroxidase activities with the substrates coniferyl alcohol, ferulic acid or indole acetic acid were compared by densitometric analysis of zymograms with those for guaiacol, and notable differences between these peroxidases in their capacity to oxidise indole acetic acid in vitro were identified. The possible functions of these peroxidases in vivo and their implications to current understanding of peroxidases in L. albus are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 14:43:09