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Titolo:
THE ROLE OF THE INTRACELLULAR AND EXTRACELLULAR SEROTONIN IN THE REGULATION OF MELATONIN PRODUCTION IN RAT PINEALOCYTES
Autore:
MIGUEZ JM; SIMONNEAUX V; PEVET P;
Indirizzi:
UNIV STRASBOURG 1,URA 1332,CNRS,12 RUE UNIV F-67000 STRASBOURG FRANCE UNIV STRASBOURG 1,URA 1332,CNRS F-67000 STRASBOURG FRANCE
Titolo Testata:
Journal of pineal research
fascicolo: 2, volume: 23, anno: 1997,
pagine: 63 - 71
SICI:
0742-3098(1997)23:2<63:TROTIA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-ACETYLTRANSFERASE ACTIVITY; CIRCADIAN-RHYTHM; GLANDS INVITRO; RELEASE; STIMULATION; 5-HYDROXYTRYPTAMINE; NOREPINEPHRINE; ACTIVATION; RECEPTORS; BLOCKADE;
Keywords:
MELATONIN; SEROTONIN SYNTHESIS AND RELEASE; TRYPTOPHAN HYDROXYLASE; SEROTONIN RECEPTORS; PINEALOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Miguez et al., "THE ROLE OF THE INTRACELLULAR AND EXTRACELLULAR SEROTONIN IN THE REGULATION OF MELATONIN PRODUCTION IN RAT PINEALOCYTES", Journal of pineal research, 23(2), 1997, pp. 63-71

Abstract

This study investigated whether the activation of pinealocyte beta-adrenergic receptors is involved in the regulation of serotonin (5-HT) synthesis and release, as it is for melatonin production. In addition, the role of the intra-and extra-cellular 5-HT in modulating the synthesis of melatonin induced by the beta-adrenergic agonist isoproterenol (ISO) was also studied. The incubation of dissociated pinealocytes with 0.1-10 mu M ISO resulted in a concentration-dependent increase of melatonin synthesis. 5-HT release and intracellular 5-HT content were increased by 0.1 and 1 mu M ISO but they were reduced after ISO 10 mu M. Moreover, when incubated with the tryptophan hydroxylase inhibitor p-chlorophenylalanine (PCPA), the secretion of 5-HT as well as the intracellular 5-HT levels were markedly reduced in both ISO-stimulated and unstimulated conditions. Melatonin release was also inhibited by PCPA,although it responded in the expected manner to increasing concentrations of ISO. These data indicate that the release of 5-HT from pinealocytes depends on the availability of cytoplasmic 5-HT which in turn ishighly dependent on the tryptophan hydroxylase activity. In cells stimulated with moderate ISO concentrations, 5-HT release may be an important regulatory process of pineal 5-HT. After a large stimulation of N-acetyltransferase (NAT) activity by ISO, the synthesis of melatonin prevails on 5-HT release, whose decrease is associated to a deficit of intracellular 5-HT. On the other hand, the present study shows that the incubation of pineal cells with high concentrations of 5-HT or with a selective 5-HT2 receptor agonist, alpha-methyl-5-hydroxytryptamine, reverses partially the inhibitory effect of PCPA on the ISO-stimulatedmelatonin synthesis. In contrast the 5-HT2 antagonist, ketanserin, results in an inhibiton of the release of melatonin following ISO stimulation, These results suggest that released 5-HT may have a role in thefull expression of the beta-adrenergically induced NAT activity and, thus, may contribute to the optimal melatonin synthesis at night.

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Documento generato il 13/07/20 alle ore 06:35:10