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Titolo:
PROSTASOME TO SPERM TRANSFER OF CD13 AMINOPEPTIDASE-N (EC-3.4.11.2)
Autore:
ARIENTI G; CARLINI E; VERDACCHI R; COSMI EV; PALMERINI CA;
Indirizzi:
IST BIOCHIM & CHIM MED,VIA GIOCHETTO I-06127 PERUGIA ITALY UNIV ROMA LA SAPIENZA,DIPARTIMENTO GINECOL & OSTET,POLICLIN UMBERTO IROME ITALY DIPARTIMENTO BIOL CELLULARE & MOL I-06127 PERUGIA ITALY
Titolo Testata:
Biochimica et biophysica acta (G). General subjects
fascicolo: 3, volume: 1336, anno: 1997,
pagine: 533 - 538
SICI:
0304-4165(1997)1336:3<533:PTSTOC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SEMINAL PLASMA; HUMAN-SEMEN; SPERMATOZOA; ORGANELLES; DIFFERENTIATION; BRADYKININ; MEMBRANES; MOTILITY; CELLS;
Keywords:
AMINOPEPTIDASE N; CD13; MEMBRANE FUSION; PH; PROSTASOME; SPERM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
G. Arienti et al., "PROSTASOME TO SPERM TRANSFER OF CD13 AMINOPEPTIDASE-N (EC-3.4.11.2)", Biochimica et biophysica acta (G). General subjects, 1336(3), 1997, pp. 533-538

Abstract

Prostasomes are membranous vesicles (150-200 nm in diameter) that arepresent in human semen. They are secreted by the prostate gland and contain large amounts of cholesterol, sphingomyelin and Ca2+. In addition, some of their proteins are enzymes. Prostasomes enhance the motility of ejaculated spermatozoa and are involved in a number of additional biological functions. In previous papers, we demonstrated that lipidcan be transferred from prostasomes to sperm by a fusion process occurring at slightly acidic pH. CD (cluster antigens) are ubiquitous proteins; in this paper, we demonstrate that CD13/aminopeptidase N is present is semen, where it is bound to prostasomes. Upon mixing prostasomes and sperm at slightly acidic pH (7 or less), aminopeptidase is transferred from prostasomes to sperm. This evidence comes from enzymatic activity determinations and from the use of the monoclonal antibody, anti-human CD13. The transfer was about 8% of total prostasomal activityat pH 5 and with a prostasome to sperm ratio of 2 (on a protein basis). The transfer did not occur at pH 8.0, but was measurable at pH 7. Therefore, this mechanism may represent a means of modifying the composition and the biological properties of ejaculated sperm. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:40:25