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Titolo:
KAINATE EXCITOTOXICITY IS MEDIATED BY AMPA-PREFERRING BUT NOT KAINATE-PREFERRING RECEPTORS IN EMBRYONIC RAT HIPPOCAMPAL CULTURES
Autore:
OHNO K; OKADA M; TSUTSUMI R; KOHARA A; YAMAGUCHI T;
Indirizzi:
YAMANOUCHI PHARMACEUT CO LTD,INST DRUG DISCOVERY RES,PHARMACOL LABS,21 MIYUKIGAOKA TSUKUBA IBARAKI 305 JAPAN YAMANOUCHI PHARMACEUT CO LTD,NEUROSCI & GASTROINTESTINAL RES LAB,INSTDRUG DISCOVERY RES TSUKUBA IBARAKI 305 JAPAN
Titolo Testata:
Neurochemistry international
fascicolo: 5, volume: 31, anno: 1997,
pagine: 715 - 722
SICI:
0197-0186(1997)31:5<715:KEIMBA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID RELEASE; D-ASPARTATE RECEPTORS; GLUTAMATE-RECEPTOR; NMDA RECEPTOR; KAINIC ACID; GYKI 52466; ALZHEIMERS-DISEASE; CEREBRAL-ISCHEMIA; NEURONAL INJURY; FOCAL ISCHEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
K. Ohno et al., "KAINATE EXCITOTOXICITY IS MEDIATED BY AMPA-PREFERRING BUT NOT KAINATE-PREFERRING RECEPTORS IN EMBRYONIC RAT HIPPOCAMPAL CULTURES", Neurochemistry international, 31(5), 1997, pp. 715-722

Abstract

We investigated kainate-induced excitotoxicity in embryonic rat hippocampal cells cultured in a chemically defined medium. Treatment with kainate for 24 h resulted in neuronal death, as assessed by the releaseof lactate dehydrogenase into the culture media. This neurotoxic effect was kainate dose-and culture age-dependent. EC50 of kainate was 127+/- 11 mu M. 2,3-dihydroxy-6-nitro-7-sulfamoylbenzo (f)quinoxaline (NBQX) completely blocked the toxicity, while MK801, an N-methyl-D-aspartate (NMDA) receptor antagonist, also blocked it but not completely. Furthermore, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) attenuated the kainate injury, while the selective and noncompetitive AMPA-preferring receptor antagonist 1-(4-aminophenyl)-4-methyl-7,8-methylene diazepine (GYKI 52466) blocked it completely. Concanavalin A (ConA), which potentiates the response to kainate at kainate-preferring receptors, had little effect on kainate toxicity. Further, AMPAalone induced little toxicity, but produced remarkable toxicity when cyclothazide was used to block the desensitization of AMPA-preferring receptors. These results indicate that kainate excitotoxicity in hippocampal cultures is mediated by AMPA- but not kainate-preferring receptors, and that it involves NMDA receptor-mediated toxicity. The non-desensitizing response at AMPA-preferring receptors may play an importantrole in kainate-induced excitotoxicity. (C) 1997 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:07:09