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Titolo:
A CONSERVED CORE ELEMENT IS FUNCTIONALLY IMPORTANT FOR MAIZE MITOCHONDRIAL PROMOTER ACTIVITY IN-VITRO
Autore:
CAOILE AGFS; STERN DB;
Indirizzi:
CORNELL UNIV,BOYCE THOMPSON INST PLANT RES,TOWER RD ITHACA NY 14853 CORNELL UNIV,BOYCE THOMPSON INST PLANT RES ITHACA NY 14853
Titolo Testata:
Nucleic acids research
fascicolo: 20, volume: 25, anno: 1997,
pagine: 4055 - 4060
SICI:
0305-1048(1997)25:20<4055:ACCEIF>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; BACTERIOPHAGE-T7 LATE PROMOTERS; YEAST MITOCHONDRIAL; RNA-POLYMERASES; NUCLEOTIDE-SEQUENCE; POINT MUTATIONS; ATP SYNTHASE; GENE; INVITRO; POTATO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
A.G.F.S. Caoile e D.B. Stern, "A CONSERVED CORE ELEMENT IS FUNCTIONALLY IMPORTANT FOR MAIZE MITOCHONDRIAL PROMOTER ACTIVITY IN-VITRO", Nucleic acids research, 25(20), 1997, pp. 4055-4060

Abstract

We have previously used a homologous in vitro transcription system todefine functional elements of the maize mitochondrial atpA promoter, These elements comprise a central domain extending from -7 to +5, relative to the transcription start site, and an upstream domain of 1-3 bpthat is purine rich and centered around positions -11 to -12. Within the central domain lies an essential 5 bp core element. These elementsare conserved in many mitochondrial promoters, but their functionality has only been tested for atpA, In this study we have introduced mutations into the corresponding elements of two cox3 promoters and show that while the core element is essential for cox3 promoter activity, upstream element mutations have little or no effect, To define the minimal sequence required for in vitro promoter activity a series of short cloned oligonucleotides corresponding to the atpA promoter was used. While some activity was seen with a 14 bp sequence, full activity required 26 bp, suggesting that elements other than the core and upstream region can influence promoter strength. Another series of clones showedthat altered spacing between the upstream and core elements of atpA had a significant effect on promoter activity, These results further define important features of the plant mitochondrial transcriptional machinery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 15:05:24