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Titolo:
DEXAMETHASONE SIGNALING IS REQUIRED TO ESTABLISH THE POSTMITOTIC STATE OF ADIPOCYTE DEVELOPMENT
Autore:
SHUGART EC; UMEK RM;
Indirizzi:
UNIV VIRGINIA,DEPT BIOL,GILMER HALL CHARLOTTESVILLE VA 22903 UNIV VIRGINIA,DEPT BIOL CHARLOTTESVILLE VA 22903
Titolo Testata:
Cell growth & differentiation
fascicolo: 10, volume: 8, anno: 1997,
pagine: 1091 - 1098
SICI:
1044-9523(1997)8:10<1091:DSIRTE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING-PROTEIN-ALPHA; GROWTH-ARREST; ECTOPIC EXPRESSION; DIFFERENTIATION; PREADIPOCYTES; PROMOTES; PROGRAM; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
E.C. Shugart e R.M. Umek, "DEXAMETHASONE SIGNALING IS REQUIRED TO ESTABLISH THE POSTMITOTIC STATE OF ADIPOCYTE DEVELOPMENT", Cell growth & differentiation, 8(10), 1997, pp. 1091-1098

Abstract

The clonal expansion phase of 3T3-L1 adipose conversion is a distinctmitotic period during which the initiation of differentiation occurs concomitant with a discrete set of mitotic divisions, During clonal expansion, a cocktail of adipogenic hormones, including the glucocorticoid dexamethasone, induced 3T3-L1 cells to progress from postconfluent adipoblasts to postmitotic adipocytes, It is reported here that expression of the growth arrest-associated gene 2 (gas2) discriminated reversible, postconfluent growth arrest from irreversible, postmitotic growth arrest, In the absence of dexamethasone, 3T3-L1 cells underwent mitoses but failed to establish postmitotic growth arrest, as evidenced by the persistence of elevated GASP mRNA, Moreover, the dexamethasone-deprived 3T3-L1 cells appeared to revert to postconfluent growth arrest, as judged by (a) their ability to reenter logarithmic growth under permissive conditions, and (b) their ability to undergo adipose conversion when subsequently challenged with a complete cocktail of adipogenic hormones, The growth potentiating factor-encoding gene, gas6, was shown to be an immediate-early target of dexamethasone, These findings reveal the requirement for dexamethasone in establishing the postmitotic state that accompanies differentiation, In addition, the results suggest that dexamethasone signaling influences the mitotic divisions of clonal expansion by determining the nature of the growth arrest state assumed upon exit from the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:54:30