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Titolo:
ENGINEERING OF A BIOLUMINESCENT ANTIGEN-BINDING PROTEIN
Autore:
BILLIALD P; MOUSLI M; GOYFFON M; VAUX D;
Indirizzi:
MUSEUM NATL HIST NAT,LERAI,57 RUE CUVIER F-75005 PARIS FRANCE UNIV OXFORD,SIR WILLIAM DUNN SCH PATHOL OXFORD OX1 3RE ENGLAND FAC PHARM TOURS F-37200 TOURS FRANCE
Titolo Testata:
Biotechnology letters
fascicolo: 10, volume: 19, anno: 1997,
pagine: 1037 - 1041
SICI:
0141-5492(1997)19:10<1037:EOABAP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; FUSION PROTEIN; IMMUNOASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
P. Billiald et al., "ENGINEERING OF A BIOLUMINESCENT ANTIGEN-BINDING PROTEIN", Biotechnology letters, 19(10), 1997, pp. 1037-1041

Abstract

A gene fusion plasmid was constructed by inserting the structural gene of the firefly luciferase into a vector which allows expression of afunctional recombinant scFv. The fusion protein which is expressed inthe periplasm of bacteria, retains dual activity and functions in bioluminescent immunoassays. Such a genetic approach offers many advantages over chemical cross-linking of antibodies to colorimetric enzymes and may be adaptable to clinical immunoassays and high through-put drugscreening assays.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 14:25:25