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Titolo:
LYN AND FGR ARE ACTIVATED IN DISTINCT MEMBRANE-FRACTIONS OF HUMAN GRANULOCYTIC CELLS
Autore:
WELCH H; MARIDONNEAUPARINI I;
Indirizzi:
CNRS,INST PHARMACOL & BIOL STRUCT,UPR 9062,205 ROUTE NARBONNE F-31077TOULOUSE FRANCE CNRS,INST PHARMACOL & BIOL STRUCT,UPR 9062 F-31077 TOULOUSE FRANCE
Titolo Testata:
Oncogene
fascicolo: 17, volume: 15, anno: 1997,
pagine: 2021 - 2029
SICI:
0950-9232(1997)15:17<2021:LAFAAI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE KINASE; ADHERENT HUMAN NEUTROPHILS; FC-GAMMA-RII; FUNCTIONAL ASSOCIATION; PLASMA-MEMBRANE; SRC; FAMILY; EXPRESSION; P58(C-FGR); RECEPTOR;
Keywords:
FGR; LYN; SRC; HCK; NEUTROPHIL; NB4 CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
H. Welch e I. Maridonneauparini, "LYN AND FGR ARE ACTIVATED IN DISTINCT MEMBRANE-FRACTIONS OF HUMAN GRANULOCYTIC CELLS", Oncogene, 15(17), 1997, pp. 2021-2029

Abstract

We have previously shown that the src-family protein-tyrosine kinase Hck is localized on the azurophil granules of human granulocytes, translocates towards the phagosomes during phagocytosis of opsonized zymosan and is activated during this process. Hck is also activated upon cell stimulation with the calcium ionophore A23187, but not with PMA or the chemotactic peptide fMLP. Here, we investigated whether the src-family kinases Lyn and Fgr are activated under the same conditions. Uponstimulation of human neutrophils or retinoic acid-differentiated NB4 cells with fMLP, only Lyn is activated. Cell stimulation with opsonized zymosan or A23187 leads to simultaneous activation of Lyn and Fgr. In cell fractionation experiments with differentiated NB4 cells, kinases show a similar the subcellular localization: Both co-fractionate quantitatively with plasma-membrane marker in two fractions that sedimentat 11000g and 200000g. Lyn is selectively activated in the 200 000 g fraction, whereas Fgr is activated in the 11 000 g fraction and distinct sets of tyrosine phosphorylated proteins are found in these fractions. The results suggest that these kinases exert their functional roles in distinct cellular compartments in human granulocytic cells.

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Documento generato il 27/11/20 alle ore 23:38:03