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Titolo:
DEVELOPMENTAL CAPACITY OF BOVINE OOCYTES FOLLOWING INHIBITION OF MEIOTIC RESUMPTION BY CYCLOHEXIMIDE OR 6-DIMETHYLAMINOPURINE
Autore:
SAEKI K; NAGAO Y; KISHI M; NAGAI M;
Indirizzi:
KINKI UNIV,COLL BIOL ORIENTED SCI & TECHNOL,DEPT GENET ENGN WAKAYAMA 64964 JAPAN SNOW BRAND MILK PROD CO LTD,EMBRYO TRANSPLANTAT LAB TOMAKOMAI HOKKAIDO 05913 JAPAN
Titolo Testata:
Theriogenology
fascicolo: 7, volume: 48, anno: 1997,
pagine: 1161 - 1172
SICI:
0093-691X(1997)48:7<1161:DCOBOF>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURE IN-VITRO; FOLLICLE-STIMULATING-HORMONE; GERMINAL VESICLE BREAKDOWN; NUCLEAR PROGRESSION; PROTEIN-SYNTHESIS; MOUSE OOCYTES; INVITRO FERTILIZATION; LUTEINIZING-HORMONE; ANTRAL FOLLICLES; CYCLIC-AMP;
Keywords:
BOVINE; INHIBITION OF MEIOTIC RESUMPTION; CYCLOHEXIMIDE; 6-DIMETHYLAMINOPURINE; DEVELOPMENTAL CAPACITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
K. Saeki et al., "DEVELOPMENTAL CAPACITY OF BOVINE OOCYTES FOLLOWING INHIBITION OF MEIOTIC RESUMPTION BY CYCLOHEXIMIDE OR 6-DIMETHYLAMINOPURINE", Theriogenology, 48(7), 1997, pp. 1161-1172

Abstract

This study was conducted to assess the fertilizability and developmental capacity of bovine oocytes which had been maintained in meiotic arrest by either a protein synthesis inhibitor, cycloheximide (CHX), or an inhibitor of serine/threonine protein kinases, 6-dimethylaminopurine (6-DMAP). Both CHX and 6-DMAP reversibly prevented nuclear maturation of nearly all oocytes for 24 h. After the reversal of arrest, CHX-treated oocytes could be successfully matured and fertilized. They developed to the blastocyst stage at slightly lower rates than oocytes cultured without inhibition for 22 h prior to sperm addition but at higherrates than those incubated in a medium containing no inhibitors for 46 h prior to fertilization. Oocytes inhibited by CHX for 48 h matured and fertilized normally but failed to develop into blastocysts. Even though 6-DMAP-treated oocytes completed meiosis I after removal from the drug, the rates of fertilization and blastocyst formation were lowerthan for untreated oocytes or CHX-treated oocytes. Effects of adding FSH and/or estradiol-17 beta (E-2) during CHX-inhibition for 24 h werealso examined. Embryos from oocytes treated with CHX and E-2 or with CHX and FSH+E-2 developed into blastocysts at similar rates as the controls. Further development of inhibited oocytes was examined by transferring blastocysts derived from oocytes inhibited by CHX with FSH and E-2 for 24 h to recipient heifers. Two calves were obtained following transfer. These results indicate that CHX-inhibited oocytes retain developmental competence, while 6-DMAP-inhibited oocytes after the reversal of arrest have reduced capacities for fertilization and further development. The addition of FSH and E-2 during CHX-inhibition improves development to the blastocyst stage of the oocytes that are capable of initiating and maintaining pregnancy after embryo transfer to recipient animals. (C) 1997 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:27:33